Recent Advances in CRISPR/Cas9-Mediated Genome Editing in Dictyostelium
Recent Advances in CRISPR/Cas9-Mediated Genome Editing in Dictyostelium
复制标题
DOI：
10.3390/cells8010046
复制
发表时间：
2019-01
期刊：
影响因子：
6
通讯作者：
Tetsuya Muramoto;Hoshie Iriki;Jun Watanabe;T. Kawata
中科院分区：
文献类型：
--
作者：
Tetsuya Muramoto;Hoshie Iriki;Jun Watanabe;T. Kawata
在过去的30年里，通过同源重组敲除靶基因的方法被广泛应用于阐明网柄苔藓中蛋白质的生理功能。最近，CRISPR/Cas9介导的基因组编辑已经成为各种生物的通用工具，包括Dictyostelials，使内源基因的快速高保真修改成为可能。在这里，我们回顾了在网柄菌基因组编辑方面的最新进展，并总结了表达sgRNA和Cas9的有用的CRISPR载体，包括几种微生物。使用这些载体，可以在2-3周内实现精确的基因组修改，从目标序列的设计开始。最后，我们讨论了CRISPR/Cas9介导的基因组编辑在网柄苔藓中的应用前景。
In the last 30 years, knockout of target genes via homologous recombination has been widely performed to clarify the physiological functions of proteins in Dictyostelium. As of late, CRISPR/Cas9-mediated genome editing has become a versatile tool in various organisms, including Dictyostelium, enabling rapid high-fidelity modification of endogenous genes. Here we reviewed recent progress in genome editing in Dictyostelium and summarised useful CRISPR vectors that express sgRNA and Cas9, including several microorganisms. Using these vectors, precise genome modifications can be achieved within 2–3 weeks, beginning with the design of the target sequence. Finally, we discussed future perspectives on the use of CRISPR/Cas9-mediated genome editing in Dictyostelium.