A monoclonal antibody against gamma-glutamyltransferase from human primary hepatoma: its use in enzyme-linked immunosorbent assay of sera of cancer patients.
A monoclonal antibody against gamma-glutamyltransferase from human primary hepatoma: its use in enzyme-linked immunosorbent assay of sera of cancer patients.
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来自人原发性肝癌的抗γ-谷氨酰转移酶的单克隆抗体：其在癌症患者血清酶联免疫吸附测定中的应用。
DOI：
10.1093/jnci/75.5.841
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
C. Sekiya
中科院分区：
文献类型：
--
作者：
N. Taniguchi;S. House;N. Kuzumaki;N. Yokosawa;S. Yamagiwa;S. Iizuka;A. Makita;C. Sekiya
从人原发性肝癌中提取了一种高纯度的γ-谷氨酰转移酶（γ-GT），并制备了γ-120单克隆抗体。免疫印迹分析表明，该抗体优先结合人肝癌和肾的γ GT的小亚基。与正常肝酶的弱结合可通过固相酶联免疫吸附测定（ELISA）检测。使用该抗体，开发了用于定量人血清中免疫反应性γ GT的ELISA。188例正常对照者的血清显示低水平（9.4 μ g/ml）的免疫反应性γ GT。原发性肝癌、转移性肝癌、胰腺癌和某些类型的肺癌患者的血清中检测到免疫反应性γ GT的水平高度升高。肝硬化患者血清中免疫反应性γ GT水平略有升高。胃肠道肿瘤患者和非恶性疾病（如肝炎和胆结石）患者血清中γ GT水平均在正常范围内。该抗体已被证明可用于诊断某些肿瘤性疾病。
A monoclonal antibody, gamma-120, was raised against a highly purified gamma-glutamyltransferase (gamma GT) from human primary hepatoma. The antibody preferentially bound to the small subunit of gamma GT from human hepatoma and kidney as evidenced by immunoblot analysis. Weak binding to the normal liver enzyme could be detected by solid-phase enzyme-linked immunosorbent assay (ELISA). With the use of this antibody, an ELISA was developed for the quantitation of immunoreactive gamma GT in human serum. Sera of 188 normal control subjects displayed a low level (9.4 micrograms/ml) of immunoreactive gamma GT. Highly elevated levels of immunoreactive gamma GT were detected in the sera of patients with primary hepatoma, metastatic liver cancer, pancreatic cancer, and certain types of lung cancer. Slightly elevated levels of immunoreactive gamma GT were seen in the sera of patients with liver cirrhosis. The levels of gamma GT were within a normal range in the sera of patients with gastrointestinal cancers and patients with nonmalignant diseases such as hepatitis and gallstones. The antibody has been shown to be useful for the diagnosis of some of the neoplastic diseases.