Zika NS1-induced ER remodeling is essential for viral replication
Zika NS1-induced ER remodeling is essential for viral replication
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Zika NS1 诱导的 ER 重塑对于病毒复制至关重要
DOI：
10.1083/jcb.201903062
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发表时间：
2020-02-03
影响因子：
7.8
通讯作者：
Shi, Lei
中科院分区：
文献类型：
--
作者：
Ci, Yali;Liu, Zhong-Yu;Shi, Lei
寨卡病毒（ZIKV）是最近出现的黄病毒家族成员，在其生命周期中在内质网形成复制区室。负责复制区室生物发生的蛋白质还没有很好地定义。本研究表明，寨卡非结构蛋白1 （NS1）诱导的内质网重塑对病毒复制至关重要。NS1在内质网腔内表达诱导内质网核周围聚集，其超微结构类似于复制室。来自模型膜系统的数据表明，NS1的膜结合和膜重塑特性取决于其疏水插入膜。这些发现表明NS1通过重塑内质网结构在黄病毒复制室形成中起着至关重要的作用。
Zika virus (ZIKV), a recently emerged member of the flavivirus family, forms replication compartments at the ER during its lifecycle. The proteins that are responsible for the biogenesis of replication compartments are not well defined. Here, we show that Zika nonstructural protein 1 (NS1)-induced ER remodeling is essential for viral replication. NS1 expressed in the ER lumen induced ER perinuclear aggregation with an ultrastructure resembling that of the replication compartment. Data from model membrane system indicated that the membrane-binding and membrane-remodeling properties of NS1 depend on its hydrophobic insertion into the membrane. These findings demonstrate that NS1 plays a crucial role in flavivirus replication compartment formation by remodeling the ER structure.