The organisation of cholesterol and ergosterol in lipid bilayers based on studies using non-perturbing fluorescent sterol probes.
The organisation of cholesterol and ergosterol in lipid bilayers based on studies using non-perturbing fluorescent sterol probes.
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基于使用非扰动荧光甾醇探针的研究，胆固醇和麦角甾醇在脂质双层中的组织。
DOI：
10.1016/0005-2736(79)90243-8
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发表时间：
1979
期刊：
影响因子：
--
通讯作者：
D. Wilton
中科院分区：
文献类型：
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作者：
J. Rogers;A. Lee;D. Wilton
研究了脱氢麦角甾醇和胆甾醇-5，7，9-三烯-3β-ol在有机溶液、水分散体中的荧光性质，并将其引入到水脂分散体中。这些探针的水分散体的吸收光谱与在有机溶液中的吸收光谱有很大的不同，并且水分散体是非荧光的。这可以归因于胶束的二聚化和/或聚集形成胶束。当类固醇被掺入脂双层时，也会发生浓度猝灭，但即使在类固醇与脂摩尔比为1：1的情况下，也能观察到相对较高的荧光。此外，在这些高摩尔比的情况下，荧光仍然是偏振的。我们将其归因于类脂双层中甾醇分子有序排列的形成。在这些阵列中，甾醇分子以端到端的方式组织，并且类脂分子防止了甾醇的面对面重叠。
The fluorescence properties of dehydroergosterol and cholesta-5,7,9-trien-3β-ol have been studied in organic solution, in aqueous dispersions and incorporated into aqueous lipid dispersions. The absorption spectra of aqueous dispersions of the probes are very different to those in organic solution, and aqueous dispersions are non-fluorescent. This can be attributed to micelle formation with dimerisation and/or aggregation in the micelles. Concentration quenching also occurs when sterols are incorporated into lipid bilayers, but relatively high fluorescence is observed even at a 1 : 1 steroid : lipid molar ratio. Further, the fluorescence is still polarized at these high molar ratios. We attribute this to the formation of ordered arrays of sterol molecules in the lipid bilayers. In these arrays the sterol molecules are organised in an end-to-end fashion, and face-to-face overlap of the sterols is prevented by the lipid molecules.