Roles of eukaryotic ribosomal proteins in maturation and transport of pre-18S rRNA and ribosome function
Roles of eukaryotic ribosomal proteins in maturation and transport of pre-18S rRNA and ribosome function
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DOI：
10.1016/j.molcel.2005.09.005
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发表时间：
2005-10-28
期刊：
影响因子：
16
通讯作者：
Milkereit, P
中科院分区：
文献类型：
--
作者：
Ferreira-Cerca, S;Pöll, G;Milkereit, P
尽管对核糖体功能和结构以及核糖体亚基如何在细菌中体外组装的知识不断增加，但许多核糖体蛋白在原核生物和真核生物中的体内作用仍然不清楚。我们对酵母核糖体蛋白（r-蛋白）小亚基的系统分析表明，大多数真核生物的r-蛋白在核糖体生物合成中扮演不同的角色，使它们对生长不可或缺。不同的r蛋白控制着细胞核和细胞质前18 S rRNA加工的不同步骤，从而确保只有正确组装的核糖体才能参与翻译。动态和稳态成熟试验的比较分析表明，需要几个R-蛋白的前18 S rRNA的有效核出口，这表明它们形成一个相互作用的平台与出口机械。相反，在核输出开始之前，主要需要其他r蛋白的存在。我们的研究绘制了r-蛋白的体外组装，结构定位和体内功能之间的相关性。
Despite the rising knowledge about ribosome function and structure and how ribosomal subunits assemble in vitro in bacteria, the in vivo role of many ribosomal proteins remains obscure both in pro- and eukaryotes. Our systematic analysis of yeast ribosomal proteins (r-proteins) of the small subunit revealed that most eukaryotic r-proteins fulfill different roles in ribosome biogenesis, making them indispensable for growth. Different r-proteins control distinct steps of nuclear and cytoplasmic pre-18S rRNA processing and, thus, ensure that only properly assembled ribosomes become engaged in translation. Comparative analysis of dynamic and steady-state maturation assays revealed that several r-proteins are required for efficient nuclear export of pre-18S rRNA, suggesting that they form an interaction platform with the export machinery. In contrast, the presence of other r-proteins is mainly required before nuclear export is initiated. Our studies draw a correlation between the in vitro assembly, structural localization, and in vivo function of r-proteins.