Analyzing mRNA-protein complexes using a yeast three-hybrid system
Analyzing mRNA-protein complexes using a yeast three-hybrid system
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DOI：
10.1016/s1046-2023(02)00015-4
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发表时间：
2002-02-01
期刊：
影响因子：
4.8
通讯作者：
Wickens, M
中科院分区：
文献类型：
--
作者：
Bernstein, DS;Buter, N;Wickens, M
RNA-蛋白质相互作用对于真核mRNA生命中每一步的正确执行和调节都是必不可少的。在这里，我们描述了一个三杂交系统中，RNA-蛋白质的相互作用，可以分析使用简单的表型或酶的测定在酿酒酵母。该系统可用于检测或确认RNA-蛋白质相互作用，从遗传学上分析RNA-蛋白质相互作用，以及在仅已知一种蛋白质或RNA伴侣时发现新的蛋白质或RNA伴侣。可以检测、鉴定和分析含有一种以上蛋白质的多组分复合物。我们描述了该方法以及如何使用它，并讨论了特别是在真核mRNA上的应用，（C）2002 Elsevier Science（美国）。All rights reserved.
RNA-protein interactions are essential for the proper execution and regulation of every step in the life of a eukaryotic mRNA. Here we describe a three-hybrid system in which RNA-protein interactions can be analyzed using simple phenotypic or enzymatic assays in Saccharomyces cerevisiae. The system can be used to detect or confirm an RNA-protein interaction, to analyze RNA-protein interactions genetically, and to discover new protein or RNA partners when only one is known. Multicomponent complexes containing more than one protein can be detected, identified, and analyzed. We describe the method and how to use it, and discuss applications that bear particularly on eukaryotic mRNAs, (C) 2002 Elsevier Science (USA). All rights reserved.