Effects of chito-oligosaccharides supplementation on growth performance, intestinal cytokine expression, autochthonous gut bacteria and disease resistance in hybrid tilapia Oreochromis niloticus ♀ x Oreochromis aureus ♂
Effects of chito-oligosaccharides supplementation on growth performance, intestinal cytokine expression, autochthonous gut bacteria and disease resistance in hybrid tilapia Oreochromis niloticus ♀ x Oreochromis aureus ♂
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DOI：
10.1016/j.fsi.2014.07.010
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发表时间：
2014-09-01
影响因子：
4.7
通讯作者：
Zhou, Zhigang
中科院分区：
文献类型：
--
作者：
Qin, Chubin;Zhang, Yuting;Zhou, Zhigang
研究了在罗非鱼(雌性×雄性)饲料中添加几丁质酶(ChiB565)--几丁质酶(ChiB565)对罗非鱼(Oreochromis Niloticus)雌性×雄性罗非鱼(Oreochromis Aureus)的生产性能、肠道免疫状况、肠道原生细菌的影响，以及对致病菌嗜水气单胞菌的保护作用。在基础日粮中添加甲壳素水解液(0.0%，T1对照；0.8%，T3；1.6%，T4；或2.4%，T5)或0.1%市售壳低聚糖作为商品推荐剂量(T2，阳性对照)，组成5个试验日粮。经过35天的饲养试验，我们发现所有处理组在罗非鱼的增重、饲料转化率和成活率方面没有显著差异。然而，在12、T3、T4和T5组，致炎蛋白肿瘤坏死因子-a和应激反应蛋白热休克蛋白70的mRNAs水平显著降低(p<；0.001)。T2组和T4组的转化生长因子-β水平较高(分别为0.001和0.0001)。此外，添加0.8%和1.6%甲壳素水解物的试验组T3和T4组的肠道细菌数(0.60<；C-5<；0.80)略有变化。在嗜水气单胞菌攻击下，壳寡糖组小鼠的死亡率低于对照组，尤其是T4组和T5组(p
We investigated the effects of incorporating chitinase (ChiB565)-hydrelyzed shrimp shell chitin into the diet of hybrid tilapia (Oreochromis niloticus female x Oreochromis aureus male) with regard to production, intestinal immune status and autochthonous gut bacteria, and protection against bacterial pathogen Aeromonas hydrophila. Five experimental diets were formulated by supplementing the basal diet with the hydrolyzed shrimp shell chitin (0.0%, T1 control; 0.8%, T3; 1.6%, T4; or 2.4%, T5) or 0.1% commercial chitosan-oligosaccharides as commercial recommendation dose (T2, positive control). After a 35-day feeding trial, we found no significant difference in weight gain, feed conversion ratio or survival rate in tilapia among all treatment groups. However, the levels of mRNAs encoding the pro-inflammatory protein tumor necrosis factor-a and the stress-response protein heat shock protein 70 were much lower in groups 12, T3, T4 and T5 (p < 0.001). The levels of transforming growth factor-beta were higher in groups T2 and T4 (p < 0.001 and p < 0.0001, respectively). In addition, group T3 and T4 with 0.8% and 1.6% hydrolyzed shrimp shell chitin supplementation respectively changed marginally their autochthonous gut bacteria (0.60 < C-5 < 0.80). When challenged with A. hydrophila, the mortality of groups fed chito-oligosaccharides was lower than the control, especially in groups T4 and T5 (p