Intraoperative diagnostics and elimination of residual microtumours with plasmonic nanobubbles
Intraoperative diagnostics and elimination of residual microtumours with plasmonic nanobubbles
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DOI：
10.1038/nnano.2015.343
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发表时间：
2016-06-01
影响因子：
38.3
通讯作者：
Lapotko, Dmitri O.
中科院分区：
文献类型：
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作者：
Lukianova-Hleb, Ekaterina Y.;Kim, Yoo-Shin;Lapotko, Dmitri O.
癌症手术未能在术中检测和消除显微镜残留病变（MRD）会导致致命的复发和转移，切除重要的正常组织会导致过度的发病率。在这里，我们表明，等离子体纳米气泡（PNB），非稳态激光脉冲激活的纳米事件，术中检测和消除MRD在手术床。PNB是在头颈部癌细胞中通过用金胶体系统靶向肿瘤并局部应用近红外、低能量短激光脉冲在体内产生的，并同时用声学探针检测。在小鼠模型中，3至30个残留癌细胞和MRD（用当前方法无法检测到）在1 ms内在手术床中以非侵入性方式检测到4 mm深。在可切除的MRD中，PNB引导的手术防止了局部复发并实现了100%的无瘤生存。在不可切除的MRD中，与标准手术相比，PNB纳米手术将生存率提高了两倍。我们的研究结果表明，PNB引导的手术和纳米手术可以在简单的术中手术中快速准确地检测和去除MRD。
Failure of cancer surgery to intraoperatively detect and eliminate microscopic residual disease (MRD) causes lethal recurrence and metastases, and the removal of important normal tissues causes excessive morbidity. Here, we show that a plasmonic nanobubble (PNB), a non-stationary laser pulse-activated nanoevent, intraoperatively detects and eliminates MRD in the surgical bed. PNBs were generated in vivo in head and neck cancer cells by systemically targeting tumours with gold colloids and locally applying near-infrared, low-energy short laser pulses, and were simultaneously detected with an acoustic probe. In mouse models, between 3 and 30 residual cancer cells and MRD (undetectable with current methods) were non-invasively detected up to 4 mm deep in the surgical bed within 1 ms. In resectable MRD, PNB-guided surgery prevented local recurrence and delivered 100% tumour-free survival. In unresectable MRD, PNB nanosurgery improved survival twofold compared with standard surgery. Our results show that PNB-guided surgery and nanosurgery can rapidly and precisely detect and remove MRD in simple intraoperative procedures.