Expression patterns and co-localization of two sensory neuron membrane proteins in Ectropis obliqua Prout, a geometrid moth pest that uses Type-II sex pheromones
Expression patterns and co-localization of two sensory neuron membrane proteins in Ectropis obliqua Prout, a geometrid moth pest that uses Type-II sex pheromones
复制标题
Ectropis obliqua Prout（一种使用 II 型性信息素的尺蛾害虫）中两种感觉神经元膜蛋白的表达模式和共定位
DOI：
10.1111/imb.12555
复制
发表时间：
2018
影响因子：
2.6
通讯作者：
Zhang Yongjun
中科院分区：
文献类型：
--
作者：
Sun Liang;Wang Qian;Zhang Yuxing;Yan Yuting;Guo Huawei;Xiao Qiang;Zhang Yongjun
感觉神经元膜蛋白 (SNMP) 作为昆虫性信息素检测的重要辅助因子。在这项研究中，我们报告了斜纹尺蠖（Ectropis obliquaProut）中的两个 SNMP，这是一种产生典型 II 型性信息素的严重尺蠖害虫。序列比对和系统发育分析表明EoblSNMP1和EoblSNMP2属于两个不同的SNMP亚家族。实时定量PCR表明EoblSNMP1偏向雄性触角，而EoblSNMP2在雄性触角上高表达，但也在雌性触角和其他化学感应组织上表达。此外，EoblSNMP1和EoblSNMP2在发育表达谱上也存在差异。原位杂交显示，EoblSNMP1是毛感器I特异的，而EoblSNMP2是在毛感器I和基本感器中表达的。此外，EoblSNMP1和EoblSNMP2在毛感器I中共表达，但在不同的细胞中。这项研究表明 EoblSNMP1 在功能上不同于 E 中的 EoblSNMP2。 obliqua;EoblSNMP1可能特别有助于性信息素的识别，而EoblSNMP2表现出多种嗅觉作用。我们的研究结果全面揭示了使用 II 型性信息素的鳞翅目物种中 SNMP 的表达模式，为 SNMP 从使用 I 型性信息素的鳞翅目蛾到使用 II 型性信息素的鳞翅目蛾的功能进化提供了新的见解。
Sensory neurone membrane proteins (SNMPs) function as essential cofactors for insect sex pheromone detection. In this study, we report two SNMPs inEctropis obliquaProut, a serious geometrid pest that produces typical Type‐II sex pheromones. Sequence alignments and phylogenetic analyses showed that EoblSNMP1 and EoblSNMP2 belong to two distinct SNMP subfamilies. Quantitative real‐time PCR suggested thatEoblSNMP1was male antennae‐biased, whereasEoblSNMP2was highly expressed on male antennae but was also expressed on female antennae and other chemosensory tissues. Additionally,EoblSNMP1andEoblSNMP2differed in their developmental expression profiles.In situhybridization revealed thatEoblSNMP1was sensilla trichodea I specific, whereasEoblSNMP2was expressed in sensilla trichodea I and the sensilla basiconica; furthermore,EoblSNMP1andEoblSNMP2were co‐expressed in sensilla trichodea I but in different cells. This study suggests thatEoblSNMP1is functionally distinct fromEoblSNMP2inE. obliqua;EoblSNMP1may specifically contribute to the recognition of sex pheromones, whereasEoblSNMP2exhibits multiple olfactory roles. Our findings comprehensively reveal the expression patterns of SNMPs in a lepidopteran species that uses Type‐II sex pheromones, providing new insights into the functional evolution of SNMPs from lepidopteran moths with Type‐I sex pheromones to those with Type‐II sex pheromones.