Interrelationships between vitamin D3 and 25-hydroxyvitamin D3 during chronic ethanol administration in the rat.
Interrelationships between vitamin D3 and 25-hydroxyvitamin D3 during chronic ethanol administration in the rat.
复制标题
大鼠慢性乙醇给药期间维生素 D3 和 25-羟基维生素 D3 之间的相互关系。
DOI：
10.1016/0026-0495(82)90028-2
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Marielle Gascon
中科院分区：
文献类型：
--
作者：
Marielle Gascon
缺乏维生素D的雌性SD大鼠以含36%总热量的乙醇作为D缺乏饲料喂养4wk，而对照组动物接受用右旋糖苷代替乙醇的等卡路里饮食。在乙醇灌胃92IU的同时，给小鼠灌胃3次，共1wk，共323wk。实验结束后，分析血浆、肝脏、横纹肌和脂肪组织中{14C}-D3和{14C}-25-羟基维生素D3的浓度。与对照组相比，乙醇处理组大鼠血浆(p<；0.02)、肌肉(p<；0.001)和脂肪组织(p<；0.001)的{14C}-D3浓度分别降低了24%、26%和59%。相比之下，血浆和肝脏中的{14C}-25(OH)D3含量分别增加了30%和55%(p<；0.001)。乙醇处理后肝脏和血浆中{14C}-25(OH)D3的增加并不伴随肌肉和脂肪组织中比例的{14C}-25(OH)D3的掺入。这些结果表明，在稳态条件下，慢性酒精摄入使25(OH)D3的产生量增加，而无变化的D3的体库显着降低。这些结果还指出，大鼠血浆中25(OH)D浓度不是确定慢性酒精摄入期间维生素D状态的可靠指标。
Vitamin D {D} depleted female Sprague-Dawley rats were fed for a period of 4 wk a D deficient diet containing 36% of total calories as ethanol while control animals received an isocaloric regimen where ethanol was substituted for by dextrins. In conjunction with the ethanol feeding 92 IU of {14 C}-vitamin D 3 {{14 C}-D 3} were administered by intragastric gavage 3 times 1 wk for 3 2 3 wk. At the end of the experiment,{14 C}-D 3 and {14 C}-25-hydroxyvitamin D 3 {{14 C}-25 (OH) D 3} concentrations were analyzed in plasma, liver, striated muscle and adipose tissue. Body reserves in unchanged {14 C}-D 3 were significantly reduced by ethanol treatment as seen by 24%, 26%, and 59% lower plasma (p< 0.02), muscle (p< 0.001) and adipose tissue (p< 0.001){14 C}-D 3 concentrations in ethanol-treated compared to control rats. In contrast total plasma and liver {14 C}-25 (OH) D 3 content were increased by 30%(p< 0.05) and 55%(p< 0.001) respectively. This increased liver and plasma {14 C}-25 (OH) D 3 following ethanol treatment was not accompanied by a proportional {14 C}-25 (OH) D 3 incorporation into muscle and adipose tissue. These results suggest that during steady state conditions 25 (OH) D 3 production is increased during chronic ethanol administration while the body pool in unchanged D 3 is significantly lowered. These results also point out that in the rat plasma 25 (OH) D concentrations are not a reliable guide for the determination of vitamin D status during chronic ethanol administration.
大鼠肝微粒体维生素 D3-25-羟化酶可溶性成分的分离和重建。
影响因子：
3.9
作者：
Yoon,PS;Deluca,HF
通讯作者：
Deluca,HF