Accumulation of an acidic dehydrin in the vicinity of the plasma membrane during cold acclimation of wheat
Accumulation of an acidic dehydrin in the vicinity of the plasma membrane during cold acclimation of wheat
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DOI：
10.1105/tpc.10.4.623
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发表时间：
1998-04-01
期刊：
影响因子：
11.6
通讯作者：
Sarhan, F
中科院分区：
文献类型：
--
作者：
Danyluk, J;Perron, A;Sarhan, F
在几种禾本科植物中发现了与抗冻性发育相关的酸性蛋白基因wcor410的表达。该基因是一个由三个同源成员wcor410、wcor410b和wcor410c组成的家族的一部分，它们已被定位于六倍体小麦同源组6染色体的长臂上。针对WCOR 410蛋白质产生抗体，并亲和纯化以消除与小麦的WCS 120类谷氨酰胺蛋白质的交叉反应性。蛋白质凝胶印迹分析显示WCOR 410蛋白质的积累与每种栽培品种的冷驯化和发展抗冻性的能力密切相关，免疫电子显微镜分析显示，这些蛋白质聚集在敏感的血管过渡区细胞质膜附近，在那里冷冻诱导的脱水可能更严重，生化分离实验表明，WCOR 410是一种外周蛋白，而不是一个完整的膜蛋白。这些结果提供了直接的证据表明，一个亚型的reclinin家族积累在质膜附近，这些蛋白质的性质，丰度和定位表明，他们参与了质膜的冷冻保护对冷冻或脱水胁迫。我们认为WCOR410在防止脱水条件下发生的质膜不稳定中起作用。
Expression of the acidic dehydrin gene wcor410 was found to be associated with the development of freezing tolerance in several Gramineae species, This gene is part of a family of three homologous members, wcor410, wcor410b, and wcor410c, that have been mapped to the long arms of the homologous group 6 chromosomes of hexaploid wheat, To gain insight into the function of this gene family, antibodies were raised against the WCOR410 protein and affinity purified to eliminate cross-reactivity with the WCS120 dehydrin-like protein of wheat, Protein gel blot analyses showed that the accumulation of WCOR410 proteins correlates well with the capacity of each cultivar to cold acclimate and develop freezing tolerance, Immunoelectron microscope analyses revealed that these proteins accumulate in the vicinity of the plasma membrane of cells in the sensitive vascular transition area where freeze-induced dehydration is likely to be more severe, Biochemical fractionation experiments indicated that WCOR410 is a peripheral protein and not an integral membrane protein. These results provide direct evidence that a subtype of the dehydrin family accumulates near the plasma membrane, The properties, abundance, and localization of these proteins suggest that they are involved in the cryoprotection of the plasma membrane against freezing or dehydration stress. We propose that WCOR410 plays a role in preventing the destabilization of the plasma membrane that occurs during dehydrative conditions.