Integration into the phage attachment site, attB, impairs multicellular differentiation in Stigmatella aurantiaca.
Integration into the phage attachment site, attB, impairs multicellular differentiation in Stigmatella aurantiaca.
复制标题
整合到噬菌体附着位点 attB 会损害橙色针头菌的多细胞分化。
DOI：
10.1128/jb.188.5.1701-1709.2006
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Kirby,JohnR
中科院分区：
文献类型：
--
作者：
Muller,Susanne;Shen,Hui;Hofmann,Diana;Schairer,HansUlrich;Kirby,JohnR
Stigmatella aurantiacadisplays a complex developmental life cycle in response to starvation conditions that results in the formation of tree-like fruiting bodies capable of producing spores. The phage Mx8, first isolated from the close relativeMyxococcus xanthus, is unable to infectS. aurantiacacells and integrate into the genome. However, plasmids containing Mx8 fragments encoding the integrase andattPare able to integrate at theattBlocus in theS. aurantiacagenome by site-specific recombination. After recombination betweenattPandattB, theS. aurantiacacells were incapable of building normal fruiting bodies but formed clumps and fungus-like structures characteristic of intermediate stages of development displayed by the wild type. We identified two tRNA genes,trnDandtrnV, encoding tRNAAspand tRNAVal, respectively, composing an operon at theattBlocus ofS. aurantiaca. Integration ofattP-containing plasmids resulted in the incorporation of the tMx8terminator sequence, in addition to a short sequence of Mx8 DNA downstream oftrnD. The integrant was unable to process thetrnDtranscript at the normal 3′ processing site and displayed a lower level of expression of thetrnVDoperon. In addition, several developmentally regulated proteins were no longer produced in mutants following insertion at theattBlocus. We hypothesize that the integration of the tMx8terminator sequence results in reduced levels of mature tRNAAspand tRNAValand that altered protein production during development is thereby responsible for the observed phenotype. ThetrnVDlocus thus defines a new developmental checkpoint forStigmatella aurantiaca.
