Identification and quantitation of auxins in plants by liquid chromatography/electrospray ionization ion trap mass spectrometry
Identification and quantitation of auxins in plants by liquid chromatography/electrospray ionization ion trap mass spectrometry
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DOI：
10.1002/rcm.3642
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发表时间：
2008-08-01
影响因子：
2
通讯作者：
Chen, Guonan
中科院分区：
文献类型：
--
作者：
Lu, Qiaomei;Zhang, Lan;Chen, Guonan
生长素是一种重要的系统激素，调节细胞的分裂、伸长和分化等特定的生理反应。建立了四种生长素的LC/ESI-ITMS联用分析方法。在最佳条件下，4种生长素(吲哚-3-乙酸、吲哚-3-丙酸、吲哚-3-丁酸和1-萘乙酸)在7min内得到完全分离和定量，最低检出限为8.0 ng mL(-1)，相对标准偏差小于5.0%。该方法还用于大白菜中生长素的分析，在自然生物基质干扰下，平均回收率在77.5%~99.8%之间。最后，我们详细讨论了已鉴定的生长素的MS相关性质。版权所有(C)2008 John Wiley&Sons，Ltd.
Auxin is an important phylohormone, which regulates specific physiological responses such as division, elongation and differentiation of cells. A new method using liquid chromatography/electrospray ionization ion trap mass spectrometry (LC/ESI-ITMS) has been developed for identification and quantitation of four auxins. Under the optimum conditions, four auxins (indole-3-acetic acid, indole-3-propionic acid, indole-3-butyric acid and 1-naphthyl acetic acid) were completely separated and quantitated within 7 min with a minimum detection limit of 8.0 ng mL(-1) with relative standard deviations lower than 5.0%. This method also has been applied to analysis of auxins in Chinese cabbage where, even with a complicated serious background perturbation due to the natural biological matrix, the mean recoveries ranged from 77.5% to 99.8%. Finally, we discuss the MS-relevant properties of the identified auxins in detail. Copyright (C) 2008 John Wiley & Sons, Ltd.