The systemic immune response of BALB/c mice infected with larval Taenia crassiceps is a mixed Th1/Th2-type response.
The systemic immune response of BALB/c mice infected with larval Taenia crassiceps is a mixed Th1/Th2-type response.
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感染粗头带绦虫幼虫的BALB/c小鼠的全身免疫反应是Th1/Th2混合型反应。
DOI：
10.1017/s0031182099004370
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发表时间：
1999
期刊：
影响因子：
2.4
通讯作者：
Kuhn,RE
中科院分区：
文献类型：
--
作者：
Toenjes,SA;Spolski,RJ;Mooney,KA;Kuhn,RE
调节实验性囊尾蚴病（粗头带绦虫）部位特异性免疫应答的淋巴细胞和细胞因子亚群尚不清楚。本研究使用流式细胞术研究了感染部位（佩奇）存在的细胞，并测量了这些细胞在感染50天内产生的细胞因子。结果显示B220+ CD 5+、B220+ CD 5 −、αβTCR+ CD 4+和CD 8+细胞扩增，同时IL-10产生短暂增加。在最初的增加之后，B220+ CD 5 −和辅助性T细胞的百分比下降，同时IL-10的产生也减少。CD 8 + T细胞在整个感染过程中持续增加，γδTCR+细胞在感染10天后增加。当用幼虫抗原刺激时，佩奇表现出在整个感染过程中IFN-γ和IL-4的产生增加。由于Th 2型极化已被证明为脾细胞从感染的BALB/c小鼠，脾细胞和佩奇在ConA和幼虫抗原的细胞因子谱进行了比较。ConA和抗原特异性刺激50天感染小鼠的脾细胞产生的IL-10的量增加，而佩奇显示IL-10的产生减少，这表明解剖学上不同的淋巴细胞群产生不同的细胞因子，并促进不同类型的反应。令人惊讶的是，在感染后期，血清中IL-4和IFN-γ的水平显著增加（分别为460倍和100倍）。因此，实验性囊尾蚴病期间BALB/c小鼠的全身免疫应答是混合的Th 1/Th 2型应答。
The subsets of lymphocytes and cytokines regulating the site-specific immune response in experimental cysticercosis (Taenia crassiceps) are not known. This study investigated the cells present at the site of infection (PECs) using flow cytometry and measured the cytokines produced by these cells through 50 days of infection. The results showed an expansion of B220+CD5+, B220+CD5−, αβTCR+CD4+ and CD8+ cells coincident with a transient increase in IL-10 production. After the initial increase, the percentage of B220+ CD5− and helper T cells decreased with a concomitant decrease in IL-10 production. CD8+ T cells continued to increase throughout infection and γδTCR+ cells increased after 10 days of infection. PECs demonstrated an increased IFN-γ and IL-4 production throughout infection when stimulated with larval antigens. Because a Th2-type polarization has been shown for spleen cells from infected BALB/c mice, cytokine profiles of spleen cells and PECs in response to ConA and larval antigens were compared. ConA and antigen-specific stimulation of spleen cells from 50-day-infected mice produced increased amounts of IL-10 while PECs showed a decreased IL-10 production suggesting that anatomically distinct lymphoid populations produce different cytokines and promote different types of responses. Surprisingly, late in infection the levels of IL-4 and IFN-γ in serum increased substantially (460-fold and 100-fold, respectively). The systemic immune response of BALB/c mice during experimental cysticercosis, therefore, is a mixed Th1/Th2-type response.