Impact of Vascular Endothelial Growth Factor on Skin Graft Survival in Irradiated Rats
Impact of Vascular Endothelial Growth Factor on Skin Graft Survival in Irradiated Rats
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DOI：
10.1001/archfacial.2008.526
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发表时间：
2009-03-01
影响因子：
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通讯作者：
Vural, Emre
中科院分区：
文献类型：
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作者：
Richter, Gresham T.;Bowen, Travis;Vural, Emre
目的：探讨血管内皮生长因子（VEGF）对全层皮肤移植（FTSG）存活的影响，并将其作为缺血条件下伤口愈合的模型。设计：24只Sprague-Dawley大鼠左背照射30 Gy （10 Gy/d）。恢复4周后，从健康的对侧背采集3厘米的ftsg，放置在照射过的受体床上。移植前，受体床在筋膜下注射VEGF蛋白（5 μ g）或生理盐水。两组间移植失败（坏死超过10%）及移植血管微血管密度比较。结果：11例经盐处理的放射床ftsg中有7例（64%）失败，而经VEGF处理的移植物失败率为23%（13例中有3例）（P= 0.048）。各组间平均微血管密度无显著差异。结论：外源性给药VEGF可改善辐照组织床FTSGs的预后。
Objective: To evaluate the effect of vascular endothelial growth factor (VEGF) on full-thickness skin graft (FTSG) survival on irradiated tissue as a model of wound healing in ischemic conditions.Design: Twenty-four Sprague-Dawley rats underwent 30 Gy of irradiation to their left dorsum (10 Gy/d). After 4 weeks of recovery, 3-cm FTSGs were harvested from the healthy contralateral dorsum and placed onto irradiated recipient beds. Before grafting, recipient beds were delivered subfascial injections of either VEGF protein (5 mu g) or physiologic saline. Graft failure (more than 10% necrosis) and graft microvascular density were compared between groups.Results: Seven of the 11 FTSGs from saline-treated irradiated beds (64%) failed, whereas the failure rate for grafts treated with VEGF was 23% (3 of 13) (P=.048). Mean microvascular density was not different between groups.Conclusion: Exogenously administered VEGF may improve the outcome of FTSGs on irradiated tissue beds.