Gamma/delta T cell antigen receptors expressed on tumor-infiltrating lymphocytes from patients with solid tumors.
Gamma/delta T cell antigen receptors expressed on tumor-infiltrating lymphocytes from patients with solid tumors.
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实体瘤患者的肿瘤浸润淋巴细胞上表达的 γ/δ T 细胞抗原受体。
DOI：
10.1002/eji.1830220310
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发表时间：
1992
影响因子：
5.4
通讯作者：
Platsoucas,CD
中科院分区：
文献类型：
--
作者：
Nanno,M;Seki,H;Mathioudakis,G;Suzuki,R;Itoh,K;Ioannides,CG;Suzuki,S;Chen,PF;Platsoucas,CD
研究了来自实体瘤患者的肿瘤浸润淋巴细胞（TIL）的T细胞系或克隆中γ/δ T细胞抗原受体（TcR）的表达。γ/δ TcR T细胞系来源于Wilms瘤、肉瘤或转移性黑色素瘤患者的TIL，通过单独的自体肿瘤细胞和重组白细胞介素2刺激，它们对自体和同种异体肿瘤细胞或K562或MEL 21肿瘤细胞系的细胞表现出非特异性细胞毒性。两种T细胞系来源于患有Wilms肿瘤的患者。其中一个使用60 kDa γ链表达非二硫键连接的γ/δ TcR，而另一个表达二硫键连接的γ/δ TcR。T细胞系来源于肉瘤患者并表达二硫键连接的γ/δ TcR，而来源于黑素瘤患者的T细胞系表达62 kDa的非二硫键连接的γ链。从转移性黑色素瘤或肾母细胞瘤患者中开发了几种T细胞克隆，并表达二硫键或非二硫键连接的γ/δ TcR。对这些克隆中的某些克隆的RNA进行北方分析显示全长γ链转录物，而α或β链转录物缺失或截短。这些T细胞克隆表现出非特异性细胞毒性。二硫键连接和非二硫键连接的TIL T细胞系和克隆均表达δ TCS 1决定簇。这些患者新鲜制备的TIL中γ/δ TcR+细胞的比例较低（<5%），其δ TCS 1/δ1比值在正常供体外周血中观察到的范围内。这些结果表明，二硫键连接和非二硫键连接的γ/δ TcR均在T细胞系和源自实体瘤TIL的克隆上表达。使用56-66-kDa γ链的非二硫键连接的γ/δ TcR经常在TIL衍生的T细胞系和克隆中发现。这些56-66-kDa的γ链很少在正常供体外周血淋巴细胞衍生的T细胞系或克隆上表达。
The expression of γ/δ T cell antigen receptors (TcR) in T cell lines or clones derived from tumor‐infiltrating lymphocytes (TIL) from patients with solid tumors was investigated. γ/δ TcR T cell lines were derived from TIL from patients with Wilms tumor, sarcoma or metastatic melanoma by stimulation with autologous tumor cells alone and recombinant interleukin 2 and they exhibited nonspecific cytotoxicity against autologous and allogeneic tumor cells, or cells of the K562 or the MEL21 tumor cell lines. Two T cell lines were derived from a patient with Wilms tumor. One of them expressed a non‐disulfide‐linked γ/δ TcR using the 60‐kDa γ chain, whereas, the other expressed a disulfide‐linked γ/δ TcR. A T cell line was derived from a patient with sarcoma and expressed a disulfide‐linked γ/δ TcR, whereas, a T cell line derived from a patient with melanoma expressed a non‐disulfide‐linked γ chain of 62 kDa. Several T cell clones were developed from patients with metastatic melanoma or Wilms tumor and expressed either disulfide‐ or non‐disulfide‐linked γ/δ TcR. Northern analysis of RNA from certain of these clones revealed a full‐length γ chain transcript, whereas, the α or β chain transcripts were either absent or truncated. These T cell clones exhibited nonspecific cytotoxicity. Both disulfide‐ and non‐disulfide‐linked TIL T cell lines and clones expressed the δ TCS1 determinant. γ/δ TcR+cells in freshly prepared TIL from these patients were present in low proportions (<5%) and their δTCS1/δ1 ratios were within the range observed in the peripheral blood of normal donors. These results demonstrate that both disulfide‐and non‐disulfide‐linked γ/δ TcR are expressed on T cell lines and clones derived from TIL from solid tumors. Non‐disulfide‐linked γ/δ TcR using the 56–66‐kDa γ chain are frequently found on TIL‐derived T cell lines and clones. These 56–66‐kDa γ chains are rarely expressed on T cell lines or clones derived from peripheral blood lymphocytes of normal donors.