Convergent solution-phase synthesis of a nucleopeptide using a protected oligonucleotide.
Convergent solution-phase synthesis of a nucleopeptide using a protected oligonucleotide.
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DOI：
10.1016/s0960-894x(99)00051-7
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发表时间：
1999-02
影响因子：
2.7
通讯作者：
D. McMinn;Marc M. Greenberg
中科院分区：
文献类型：
--
作者：
D. McMinn;Marc M. Greenberg
通过保护的生物聚合物在溶液中的链段偶联，以收敛的方式制备了核肽。仅用与寡核苷酸相当的5个等价物即可得到含有λ抑制物结合部位的核多肽(4b，72%)和含有螺旋转角蛋白结合螺旋的共同序列的多肽。这表明，最近发展的受保护寡核苷酸的溶液相偶联方法适用于具有潜在二级结构的较大核多肽的收敛合成。
A nucleopeptide was prepared in a convergent manner via segmental coupling of the protected biopolymers in solution. The resulting nucleopeptide (4b, 72%) containing the binding site of λ repressor and a peptide containing the consensus sequence of the DNA binding helix of the helix turn-helix-proteins was obtained using only five equivalents of the peptide relative to the oligonucleotide. This demonstrates that the recently developed method for the solution phase coupling of protected oligonucleotides is amenable to the convergent synthesis of larger nucleopeptides that are potentially capable of adopting secondary structure.