The midgut bacterial flora of wild Aedes triseriatus, Culex pipiens, and Psorophora columbiae mosquitoes
The midgut bacterial flora of wild Aedes triseriatus, Culex pipiens, and Psorophora columbiae mosquitoes
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DOI：
10.4269/ajtmh.1996.54.219
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发表时间：
1996-02-01
影响因子：
3.3
通讯作者：
Beier, JC
中科院分区：
文献类型：
--
作者：
DeMaio, J;Pumpuni, CB;Beier, JC
对野外捕获的三列伊蚊、尖音库蚊和柱状痒螨中肠植物群进行了调查。解剖中肠内容物进行了检查，使用定量需氧细菌培养。对单个菌落（n = 134）进行传代培养并鉴定为种属。中肠细菌数量在蚊子发育过程中发生了巨大变化。在幼虫阶段和蛹出现之间，细菌数量减少了280- 1，100倍，而在吸血后，细菌数量增加了70- 16，000倍。细菌鉴定揭示了一个复杂的植物群与多达9个属，在任何发展阶段确定。最常分离的菌种为粘质沙雷氏菌、臭氧克雷伯氏菌、铜绿假单胞菌和成团肠杆菌。在蚊子中肠植物群中存在遗传上充分表征的细菌可能提供在媒介物种中表达新的遗传产物的手段。
The midgut bacterial flora of wild-caught Aedes triseriatus, Culex pipiens, and Psorophora columbiae mosquitoes was investigated. Dissected midgut contents were examined using quantitative aerobic bacterial cultures. Individual colonies (n = 134) were subcultured and identified to species. Midgut bacterial counts changed dramatically during mosquito development. A 280-1,100-fold decrease in the bacterial population occurred between the larval stage and pupal emergence, whereas a subsequent 70-16,000-fold increase occurred after blood-feeding. Bacterial identifications revealed a complex flora with up to nine genera identified during any stage of development. Species most frequently isolated were Serratia marcescens, Klebsiella ozonae, Pseudomonas aeruginosa, and Enterobacter agglomerans. The presence of genetically well-characterized bacteria in the midgut flora of mosquitoes may provide a means of expressing novel genetic products in vector species.