Safety, Protective Immunity, and DIVA Capability of a Rough Mutant Salmonella Pullorum Vaccine Candidate in Broilers.
Safety, Protective Immunity, and DIVA Capability of a Rough Mutant Salmonella Pullorum Vaccine Candidate in Broilers.
复制标题
肉鸡粗突变型白痢沙门氏菌候选疫苗的安全性、保护性免疫和 DIVA 能力
DOI：
10.3389/fmicb.2017.00547
复制
发表时间：
2017
影响因子：
5.2
通讯作者：
Jiao X
中科院分区：
文献类型：
--
作者：
Guo R;Jiao Y;Li Z;Zhu S;Fei X;Geng S;Pan Z;Chen X;Li Q;Jiao X
肠沙门氏菌亚种鸡鸡肠杆菌白痢沙门氏菌（Salmonella Pullorum）高度适应鸡，引起急性全身性疾病，导致高死亡率。疫苗接种是促进动物健康、食品安全和减少沙门氏菌控制环境持久性的一种方法。一个重要的考虑因素是家禽沙门氏菌疫苗接种不应干扰沙门氏菌病监测计划。这是 DIVA（区分受感染和已接种疫苗的动物）计划的基础。为了实现这一目标，在之前开发的 spiC 突变体的基础上开发了 waaL 突变体。该候选疫苗（白痢沙门氏菌 ΔspiCΔwaaL）的安全性、有效性和 DIVA 特征在肉鸡中进行了评估。我们的结果表明，疫苗株中截短的 LPS 具有区分用途，既可以作为细菌学标记（粗略表型），也可以作为血清学标记，有助于区分感染鸡和接种鸡。粗糙突变体表现出足够的安全性，对宿主雏鸡无毒，并且对环境压力表现出更高的敏感性。用突变体对日龄肉鸡进行单次肌肉内免疫，通过显着刺激体液和细胞免疫反应以及减少攻击菌株的定植，提供针对致命野生型攻击的理想保护。与对照组相比，接种组的平均病理学评分显着降低。此外，突变菌株产生了针对野生型鸡沙门氏菌攻击的交叉保护，从而提高了存活率，并且与野生型肠炎沙门氏菌产生了交叉保护，从而减少了定植。这些结果表明，双突变株可能是一种安全、有效、交叉保护的疫苗，可预防雏鸡沙门氏菌感染，同时符合 DIVA 计划的要求。
Salmonella enterica subsp. enterica serovar Gallinarum biovar Pullorum (Salmonella Pullorum) is highly adapted to chickens causing an acute systemic disease that results in high mortality. Vaccination represents one approach for promoting animal health, food safety and reducing environmental persistence in Salmonella control. An important consideration is that Salmonella vaccination in poultry should not interfere with the salmonellosis monitoring program. This is the basis of the DIVA (Differentiation of Infected and Vaccinated Animals) program. In order to achieve this goal, waaL mutant was developed on a spiC mutant that was developed previously. The safety, efficacy, and DIVA features of this vaccine candidate (Salmonella Pullorum ΔspiCΔwaaL) were evaluated in broilers. Our results show that the truncated LPS in the vaccine strain has a differentiating use as both a bacteriological marker (rough phenotype) and also as a serological marker facilitating the differentiation between infected and vaccinated chickens. The rough mutant showed adequate safety being avirulent in the host chicks and showed increased sensitivity to environmental stresses. Single intramuscular immunization of day-old broiler chicks with the mutant confers ideal protection against lethal wild type challenge by significantly stimulating both humoral and cellular immune responses as well as reducing the colonization of the challenge strain. Significantly lower mean pathology scores were observed in the vaccination group compared to the control group. Additionally, the mutant strain generated cross-protection against challenge with the wild type Salmonella Gallinarum thereby improving survival and with the wild type Salmonella Enteritidis thereby reducing colonization. These results suggest that the double-mutant strain may be a safe, effective, and cross-protective vaccine against Salmonella infection in chicks while conforming to the requirements of the DIVA program.