Functional complementation by electroporation of human BACs into mammalian fibroblast cells
Functional complementation by electroporation of human BACs into mammalian fibroblast cells
复制标题
DOI：
10.1093/nar/26.4.1124
复制
发表时间：
1998-02-15
影响因子：
14.9
通讯作者：
Moses, RE
中科院分区：
文献类型：
--
作者：
Hejna, JA;Johnstone, PL;Moses, RE
细菌人工染色体（BACs）已被用于补充代谢缺陷，并通过电穿孔将耐药性标记物转移到哺乳动物细胞中。荧光原位杂交、PCR和Southern杂交显示，选择性标记稳定，受体细胞已将BAC DNA整合到染色体中。
Bacterial Artificial Chromosomes (BACs) have been used to complement a metabolic defect and to transfer a drug resistance marker into mammalian cells by electroporation. The selectable markers are stable and the recipient cells have BAC DNA integrated into the chromosomes as shown by fluorescent in situ hybridization, PCR and Southern hybridization.