Highly passage of Spodoptera litura cell line causes its permissiveness to baculovirus infection
Highly passage of Spodoptera litura cell line causes its permissiveness to baculovirus infection
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DOI：
10.1007/s10616-008-9158-8
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发表时间：
2008-08
期刊：
影响因子：
2.2
通讯作者：
Xuping Zhang;Wenjie Lan;Yu-jie Deng;Yuan Ma;Kaiyu Liu;Jianxin Peng;Yi Li;H. Hong
中科院分区：
文献类型：
--
作者：
Xuping Zhang;Wenjie Lan;Yu-jie Deng;Yuan Ma;Kaiyu Liu;Jianxin Peng;Yi Li;H. Hong
It is well known that the characteristics of cell lines possibly alter when cell lines are at high-passage number because of the environmental selection. We do not know whether non-permissive or low-permissive cell lines could become permissive or more permissive to virus infection after over-high passage. In the present studies, the alteration of the permissiveness ofSpodoptera lituracell line Sl-zsu-1 to three baculovirus infection was investigated after over-high passage, and the possible mechanisms are also investigated. Vigorous apoptosis in Sl-zsu-1 cells was induced by both the recombinantAutographa californicamultiple nucleopolyhedrovirus AcMNPV-GFP-actin and the celery looperAnagrapha falciferamultiple nucleopolyhedrovirus AfMNPV, suggesting the replication of the two viruses was blocked by apoptosis. However, the cells infected byS. lituramulticapsid nucleopolyhedrovirus SpltMNPV did not undergo apoptosis, but the SpltMNPV titre of the supernatant was not detectable, suggesting this cell line was low-permissive for this virus infection and other factor(s) involved in blockage of the virus replication except apoptosis. However, when Sl-zsu-1 cells had been subcultured continuously for more than 4 years (high-passage cell), which was named as Sl-HP cell line afterwards, no significant apoptosis was induced by the three baculovirus in Sl-HP cells, and many replicated virions or nucleocapsids were observed in the cells. But the permissiveness of Sl-HP cells to the three viruses was very different according to the titre of viruses in the cell cultures. Interestingly, the DNA extracted from SpltMNPV could induce vigorous apoptosis of Sl-HP cells. Altogether, Sl-zsu-1 cell line becomes more permissive to baculovirus infection after over-high passage and multiple paths can block the baculovirus infectivity.
