Altered Hepa1-6 cells by dimethyl sulfoxide (DMSO)-treatment induce anti-tumor immunity in vivo.
Altered Hepa1-6 cells by dimethyl sulfoxide (DMSO)-treatment induce anti-tumor immunity in vivo.
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二甲亚砜 (DMSO) 处理改变的 Hepa1-6 细胞可诱导体内抗肿瘤免疫
DOI：
10.18632/oncotarget.7009
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发表时间：
2016-02-23
期刊：
影响因子：
--
通讯作者：
Zhu H
中科院分区：
文献类型：
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作者：
Jiang Z;Zhang H;Wang Y;Yu B;Wang C;Liu C;Lu J;Chen F;Wang M;Yu X;Lin J;Pan X;Wang P;Zhu H
癌症免疫疗法是利用免疫系统来治疗癌症。本研究提出了激活免疫系统参与肿瘤免疫治疗的可选策略。当用2%DMSO处理Hepa 1 -6细胞时，细胞增殖受到抑制，没有明显的凋亡或活力降低。D-hep细胞，即Hepa 1 -6细胞，用DMSO处理7天，在不含DMSO的培养基中可以恢复到较高的增殖率，但基因表达谱的改变是不可逆的。有趣的是，来自D-hep细胞而不是Hepa 1 -6细胞的肿瘤在野生型C57 BL/6小鼠中消退，而D-hep细胞在免疫缺陷小鼠中表现出与Hep 1 -6细胞相似的肿瘤发生。正如预期的那样，额外的Hepa 1 -6细胞在D-hep-C57小鼠中未能形成肿瘤，其中D-hep细胞被消除。进一步的研究证实，D-hep-C57小鼠建立了针对Hepa 1 -6细胞的抗肿瘤免疫。我们的研究表明，DMSO处理的具有改变的生物学特性的活的肿瘤细胞可以在体内诱导抗肿瘤免疫。
Cancer immunotherapy is the use of the immune system to treat cancer. Our current research proposed an optional strategy of activating immune system involving in cancer immunotherapy. When being treated with 2% DMSO in culture medium, Hepa1-6 cells showed depressed proliferation with no significant apoptosis or decreased viability. D-hep cells, Hepa1-6 cells treated with DMSO for 7 days, could restore to the higher proliferation rate in DMSO-free medium, but alteration of gene expression profile was irreversible. Interestingly, tumors from D-hep cells, not Hepa1-6 cells, regressed in wild-type C57BL/6 mice whereas D-hep cells exhibited similar tumorigenesis as Hep1–6 cells in immunodeficient mice. As expected, additional Hepa1-6 cells failed to form tumors in the D-hep-C57 mice in which D-hep cells were eliminated. Further research confirmed that D-hep-C57 mice established anti-tumor immunity against Hepa1-6 cells. Our research proposed viable tumor cells with altered biological features by DMSO-treatment could induce anti-tumor immunity in vivo.