Two-Dimensional N-Glycan Distribution Mapping of Hepatocellular Carcinoma Tissues by MALDI-Imaging Mass Spectrometry.
Two-Dimensional N-Glycan Distribution Mapping of Hepatocellular Carcinoma Tissues by MALDI-Imaging Mass Spectrometry.
复制标题
通过MALDIMIMIGAGE质谱法对肝细胞癌组织的二维N-聚糖分布图。
DOI：
10.3390/biom5042554
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发表时间：
2015-10-15
期刊：
影响因子：
5.5
通讯作者：
Drake RR
中科院分区：
文献类型：
--
作者：
Powers TW;Holst S;Wuhrer M;Mehta AS;Drake RR
最近开发了一种新的质谱成像方法，可以同时绘制组织中N-聚糖的二维分布。该方法使用基质辅助激光解吸电离成像质谱（MALDI-IMS）对冷冻或福尔马林固定组织中肽N-糖苷酶F释放的多个N-连接聚糖物质的位置和分布进行空间分析。用该方法评价了多个福尔马林固定的人肝细胞癌组织，检测到一组超过30种N-聚糖。对从相邻载玻片中释放的提取N-聚糖进行乙基化反应，以稳定含唾液酸的聚糖，并将这些结构与直接从组织分析中检测到的N-聚糖进行比较。此外，将肿瘤组织微阵列核心中检测到的单岩藻糖基化N-聚糖的分布与核心岩藻糖结合凝集素的组织化学染色模式进行比较。由于这种MALDI-IMS工作流程有可能应用于任何福尔马林固定的组织块或组织微阵列，该技术的优点和局限性，在上下文中与其他糖组学方法也进行了总结。
A new mass spectrometry imaging approach to simultaneously map the two-dimensional distribution of N-glycans in tissues has been recently developed. The method uses Matrix Assisted Laser Desorption Ionization Imaging Mass Spectrometry (MALDI-IMS) to spatially profile the location and distribution of multiple N-linked glycan species released by peptide N-glycosidase F in frozen or formalin-fixed tissues. Multiple formalin-fixed human hepatocellular carcinoma tissues were evaluated with this method, resulting in a panel of over 30 N-glycans detected. An ethylation reaction of extracted N-glycans released from adjacent slides was done to stabilize sialic acid containing glycans, and these structures were compared to N-glycans detected directly from tissue profiling. In addition, the distribution of singly fucosylated N-glycans detected in tumor tissue microarray cores were compared to the histochemistry staining pattern of a core fucose binding lectin. As this MALDI-IMS workflow has the potential to be applied to any formalin-fixed tissue block or tissue microarray, the advantages and limitations of the technique in context with other glycomic methods are also summarized.