Comparative Colocalization Single-Molecule Spectroscopy (CoSMoS) with Multiple RNA Species
Comparative Colocalization Single-Molecule Spectroscopy (CoSMoS) with Multiple RNA Species
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DOI：
10.1007/978-1-0716-0278-2_3
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发表时间：
2020-01-01
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影响因子：
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通讯作者：
Braun, Joerg E.
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作者：
Haraszti, Reka A.;Braun, Joerg E.
共定位单分子光谱 (CoSMoS) 允许以单分子分辨率研究细胞环境的全部复杂性中的 RNA-蛋白质复合物。传统上，单一 RNA 种类与多种蛋白质之间的相互作用是实时监测的。然而，比较同一细胞提取物中相同蛋白质与不同RNA种类的相互作用可以为RNA生物学提供独特的见解。在这里，我们描述了一种同时监测多种 RNA 物种以实现直接比较的方法。这种方法代表了避免传统实验间比较的技术发展。
Colocalization single-molecule spectroscopy (CoSMoS) allows studying RNA-protein complexes in the full complexity of their cellular environment at single-molecule resolution. Conventionally, the interaction between a single RNA species and multiple proteins is monitored in real time. However, comparing interactions of the same proteins with different RNA species in the same cell extract promises unique insights into RNA biology. Here, we describe an approach to monitor multiple RNA species simultaneously to enable direct comparison. This approach represents a technological development to avoid conventional inter-experiment comparisons.