Simultaneous transfer of cholesteryl ester and phospholipid by protein(s) isolated from human lipoprotein-free plasma.
Simultaneous transfer of cholesteryl ester and phospholipid by protein(s) isolated from human lipoprotein-free plasma.
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通过从人无脂蛋白血浆中分离出的蛋白质同时转移胆固醇酯和磷脂。
DOI：
10.1016/0006-291x(80)90604-x
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发表时间：
1980
影响因子：
3.1
通讯作者：
R. Jackson
中科院分区：
文献类型：
--
作者：
J. Ihm;J. Harmony;J. Ellsworth;R. Jackson
催化[~ 3 H]胆固醇酯和[~（14）C]-磷脂酰胆碱从高密度脂蛋白向低密度脂蛋白转移的蛋白质已从人血浆中纯化了4829倍，方法是将d > 1.21 μ g/ml的人血浆下层馏分在苯基-琼脂糖、CM-纤维素、伴刀豆球蛋白A-Sepharose上层析，最后通过等电聚焦。在纯化的每一步，两种转移活性都是一致的。分子量为150，000的纯化蛋白质以1：1的化学计量和相等的流量转移胆固醇酯和磷脂酰胆碱。大鼠血浆中含有一种蛋白质，可与人血浆一样有效地促进磷脂酰胆碱的转移。然而，大鼠血浆蛋白不促进胆固醇酯的转移。这些结果表明，人血浆中含有一种或多种蛋白质，可能作为1：1复合物转移两种脂质，而大鼠血浆中缺乏胆固醇酯转移蛋白。
Protein(s) catalyzing the transfer of [3H]cholesteryl ester and [14C]-phosphatidylcholine from high density lipoproteins to low density lipoproteins have been purified 4829-fold from human plasma by chromatography of the d > 1.21 g/ml infranatant fraction of human plasma on phenyl-Sepharose, CM-cellulose, concanavalin A-Sepharose, and finally by isoelectric focussing. At each step of the purification, both transfer activities coelute. The purified protein(s), molecular weight 150,000, transfer cholesteryl esters and phosphatidylcholine with a 1:1 stoichiometry and at equal rates of flux. Rat plasma contains a protein(s) which facilitates the transfer of phosphatidylcholine as effectively as human plasma. However, the rat plasma protein(s) does not facilitate the transfer of cholesteryl esters. These results suggest that human plasma contains one or more proteins which transfer both lipids, possibly as a 1:1 complex, whereas rat plasma lacks the cholesteryl ester transfer protein.