Recombinant protein production in a variety of Nicotiana hosts: a comparative analysis
Recombinant protein production in a variety of Nicotiana hosts: a comparative analysis
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DOI：
10.1111/j.1467-7652.2010.00563.x
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发表时间：
2011-05-01
影响因子：
13.8
通讯作者：
Menassa, Rima
中科院分区：
文献类型：
--
作者：
Conley, Andrew J.;Zhu, Hong;Menassa, Rima
虽然许多不同的作物品种已被用于生产各种疫苗、抗体、生物制药和工业酶，但烟草在生产重组蛋白方面的历史最为悠久。为了进一步提高烟草平台的外源蛋白产量，研究了四种重组蛋白（人促红细胞生成素和白细胞介素-10、抗铜绿假单胞菌抗体和超耐热α -淀粉酶）在多个烟草品种和品种中的瞬时稳定表达。重组蛋白的瞬时积累水平在不同烟叶寄主间差异显著，而烟叶品种对稳定转基因植株中重组蛋白的积累水平影响不大。此外，本研究还检测了不同烟叶品种的生长速率、叶片生物量、总可溶性蛋白水平和生物碱含量，以建立重组蛋白商业化生产的最佳植物平台。在被评估的52个烟草品种中，烟草(cv。i64)除了产生大量的生物量和相对较少的生物碱外，还产生了最高的瞬时浓度的重组蛋白，这可能使其成为生产重组蛋白最有效的植物宿主。
P>Although many different crop species have been used to produce a wide range of vaccines, antibodies, biopharmaceuticals and industrial enzymes, tobacco has the most established history for the production of recombinant proteins. To further improve the heterologous protein yield of tobacco platforms, transient and stable expression of four recombinant proteins (i.e. human erythropoietin and interleukin-10, an antibody against Pseudomonas aeruginosa, and a hyperthermostable alpha-amylase) was evaluated in numerous species and cultivars of Nicotiana. Whereas the transient level of recombinant protein accumulation varied significantly amongst the different Nicotiana plant hosts, the variety of Nicotiana had little practical impact on the recombinant protein concentration in stable transgenic plants. In addition, this study examined the growth rate, amount of leaf biomass, total soluble protein levels and the alkaloid content of the various Nicotiana varieties to establish the best plant platform for commercial production of recombinant proteins. Of the 52 Nicotiana varieties evaluated, Nicotiana tabacum (cv. I 64) produced the highest transient concentrations of recombinant proteins, in addition to producing a large amount of biomass and a relatively low quantity of alkaloids, probably making it the most effective plant host for recombinant protein production.