Functional CpG methylation system in a social insect
Functional CpG methylation system in a social insect
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DOI：
10.1126/science.1135213
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发表时间：
2006-10-27
期刊：
影响因子：
56.9
通讯作者：
Robinson, Gene E.
中科院分区：
文献类型：
--
作者：
Wang, Ying;Jorda, Mireia;Robinson, Gene E.
DNA甲基化系统在脊椎动物中有很好的特征，但在黑腹果蝇和其他无脊椎动物中甲基化仍然存在争议。利用最近测序的蜜蜂基因组，我们提出了昆虫功能DNA甲基化系统的生物信息学，分子和生化表征。我们报道了脊椎动物DNA甲基转移酶Dnmt1和Dnmt3a和b的催化活性同源物，这两种同种异构体含有甲基DNA结合域，基因组5-甲基脱氧胞嘧啶和cpg甲基化基因。蜜蜂为研究甲基化在社会环境中的作用提供了一个机会。
DNA methylation systems are well characterized in vertebrates, but methylation in Drosophila melanogaster and other invertebrates remains controversial. Using the recently sequenced honey bee genome, we present a bioinformatic, molecular, and biochemical characterization of a functional DNA methylation system in an insect. We report on catalytically active orthologs of the vertebrate DNA methyltransferases Dnmt1 and Dnmt3a and b, two isoforms that contain a methyl-DNA binding domain, genomic 5-methyl-deoxycytosine, and CpG-methylated genes. The honey bee provides an opportunity to study the roles of methylation in social contexts.