Immune transfer protects severely immunosuppressed mice from murine cytomegalovirus retinitis and reduces the viral load in ocular tissue
Immune transfer protects severely immunosuppressed mice from murine cytomegalovirus retinitis and reduces the viral load in ocular tissue
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DOI：
10.1086/315781
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发表时间：
2000-09-01
影响因子：
6.4
通讯作者：
Mitchell, BM
中科院分区：
文献类型：
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作者：
Kercher, L;Mitchell, BM
巨细胞病毒(CMV)视网膜炎是一种影响视力的疾病，影响免疫抑制的人，在艾滋病患者中很常见。本研究的目的是评估小鼠巨细胞病毒(MCMV)视网膜炎的补充性模型，过继免疫转移到严重免疫抑制的动物。用环磷酰胺免疫抑制的成年BALB/c小鼠，视网膜下接种5×10(2)个MCMV空斑形成单位，4~6小时后，静脉输注3~4×10(7)个供体细胞。移植8天后，对接受供体细胞的小鼠进行组织学研究、感染性病毒滴定，并进行聚合酶链式反应(PCR)分析，过继转移总MCMV免疫淋巴(LN)细胞或浓缩的LN淋巴细胞，即使在感染开始后也能显著保护免疫抑制的小鼠免受视网膜炎的侵袭。通过空斑分析和聚合酶链式反应检测，转移导致了病毒载量的降低。这一补充模型将有助于确定CMV视网膜炎保护的特异性免疫参数。
Cytomegalovirus (CMV) retinitis is a sight-threatening disease that affects immunosuppressed people and is prevalent in people with AIDS. The purpose of this study was to evaluate murine CMV (MCMV) retinitis in a replenishing model with adoptive immune transfer into severely immunosuppressed animals. Adult BALB/c mice, immunosuppressed with cyclophosphamide, were infected subretinally with 5 x 10(2) plaque-forming units of MCMV, Four to six hours later, 3-4 x 10(7) donor cells were transferred by intravenous infusion. Eight days after the transfer, the eyes that had received donor cells were studied histologically, titered for infectious virus, and analyzed with polymerase chain reaction (PCR), Adoptive transfer of total MCMV-immune lymph node (LN) cells or enriched LN lymphocytes specifically and significantly protected immunosuppressed mice from retinitis even after the initiation of infection. The transfer resulted in a reduced viral load, as measured by both plaque assay and PCR. This replenishment model will be useful for determining the specific immune parameters of protection from CMV retinitis.