Surface carbohydrate analysis and bioethanol production of sugarcane bagasse pretreated with the white rot fungus, Ceriporiopsis subvermispora and microwave hydrothermolysis
Surface carbohydrate analysis and bioethanol production of sugarcane bagasse pretreated with the white rot fungus, Ceriporiopsis subvermispora and microwave hydrothermolysis
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DOI：
10.1016/j.biortech.2011.07.027
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发表时间：
2011-11-01
影响因子：
11.4
通讯作者：
Watanabe, Takashi
中科院分区：
文献类型：
--
作者：
Sasaki, Chizuru;Takada, Rie;Watanabe, Takashi
研究了白色腐菌Ceriporiopsis subvermispora预处理和微波水热处理对甘蔗渣酶法糖化发酵的影响。最好的糖产量，44.9克每100克的甘蔗渣得到真菌处理，然后在180摄氏度的微波水热处理20分钟。荧光标记的碳水化合物结合模块，识别结晶纤维素（CjCBM 3-GFP），非结晶纤维素（CjCBM 28-GFP）和木聚糖（CtCBM 22-GFP）被施加到表征暴露的多糖。有和没有真菌培养的微波预处理导致类似水平的纤维素暴露，但组合处理引起更多的纤维分离和植物组织变薄。同时糖化和发酵的纸浆馏分通过微波水热法与真菌处理和不处理，得到乙醇产率分别为35.8%和27.0%，基于综纤维素在纸浆中的含量。这些结果表明C. subvermispora预处理可能是从甘蔗渣生产乙醇的过程的有益部分。(C)2011年由Elsevier Ltd.出版
Effects of pretreatments with a white rot fungus, Ceriporiopsis subvermispora, and microwave hydrothermolysis of bagasse on enzymatic saccharification and fermentation were evaluated. The best sugar yield, 44.9 g per 100 g of bagasse was obtained by fungal treatments followed by microwave hydrothermolysis at 180 degrees C for 20 min. Fluorescent-labeled carbohydrate-binding modules which recognize crystalline cellulose (CjCBM3-GFP), non-crystalline cellulose (CjCBM28-GFP) and xylan (CtCBM22-GFP) were applied to characterize the exposed polysaccharides. The microwave pretreatments with and without the fungal cultivation resulted in similar levels of cellulose exposure, but the combined treatment caused more defibration and thinning of the plant tissues. Simultaneous saccharification and fermentation of the pulp fractions obtained by microwave hydrothermolysis with and without fungal treatment, gave ethanol yields of 35.8% and 27.0%, respectively, based on the holocellulose content in the pulp. These results suggest that C. subvermispora pretreatment could be beneficial part of the process to produce ethanol from bagasse. (C) 2011 Published by Elsevier Ltd.