Mechanism of autonomous control of the Escherichia coli F plasmid: purification and characterization of the repE gene product.
Mechanism of autonomous control of the Escherichia coli F plasmid: purification and characterization of the repE gene product.
复制标题
大肠杆菌 F 质粒的自主控制机制：repE 基因产物的纯化和表征。
DOI：
10.1093/nar/16.2.413
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发表时间：
1988
影响因子：
14.9
通讯作者：
Ray,DS
中科院分区：
文献类型：
--
作者：
Masson,L;Ray,DS
E蛋白是29 KdrepE基因的产物，是大肠杆菌F质粒复制所必需的。克隆了E基因，并在诱导型ATG融合载体中表达，蛋白产物已纯化至均一。纯化的E蛋白在溶液中以二聚体形式存在，并特异性结合在复制ori 2和复制操纵基因DNA的mini-F起点内发现的19-bp直接重复序列（incB）。对E蛋白氨基酸序列的检查揭示了DNA结合的共有序列。
E protein, the 29 KdrepEgene product, is essential for the replication of theEscherichia coliF plasmid. TherepEgene has been cloned and expressed in an inducible ATG-fusion vector, and the protein product has been purified to homogeneity. The purified E protein is present as a dimer in solution and binds specifically to both the 19-bp direct repeats (incB) found within the mini-F origin of replicationori2and therepEoperator DNA. Examination of the ami no acid sequence of E protein revealed a consensus sequence for DNA binding.