XMRV Infection in Patients With Prostate Cancer: Novel Serologic Assay and Correlation With PCR and FISH
XMRV Infection in Patients With Prostate Cancer: Novel Serologic Assay and Correlation With PCR and FISH
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DOI：
10.1016/j.urology.2010.01.038
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发表时间：
2010-04-01
期刊：
影响因子：
2.1
通讯作者：
Petros, John A.
中科院分区：
文献类型：
--
作者：
Arnold, Rebecca S.;Makarova, Natalia V.;Petros, John A.
目的：建立一种血清中和抗体检测方法，用于检测异嗜性鼠白血病病毒相关病毒（XMRV）逆转录病毒的中和抗体，并将该方法与聚合酶链反应和荧光原位杂交技术相结合，用于鉴别前列腺癌患者是否感染过XMRV病毒，以及前列腺中是否携带XMRV病毒序列。并在手术中获得生物标本。使用TaqMan基因分型试验对来自外周血的DNA对患者进行R462Q RNASEL变体的基因分型。开发了一种检测XMRV中和抗体的血清测定法，并用于确定哪些患者有既往感染XMRV病毒的血清学证据。还使用聚合酶链反应和荧光原位杂交分析来测试这些患者中的一些患者的前列腺中XMRV核苷酸序列的存在。150个，我们的分析检测到11个在40例携带XMRV中和抗体的患者中，有8例（27.5%）携带RNASEL基因型QQ，3例（15%）携带RQ或RR基因型。这些结果与其他2个检测（聚合酶链反应和荧光原位杂交），旨在检测XMRV infection.Conclusions XMRV感染一些前列腺癌患者是完全一致的。抗XMRV中和抗体与2种独立的前列腺病毒检测方法相关。抗体反应表明，随着临床血清学检测的发展，有可能筛查患者的XMRV感染。本报告中列出的病例提供了可用于开发临床相关测定的生物样本。泌尿学75：755 - 761，2010年。爱思唯尔公司出版
OBJECTIVES To develop a serum-based assay to detect neutralizing antibodies to the xenotropic murine leukemia virus-related virus (XMRV) retrovirus and to use this assay with polymerase chain reaction and fluorescence in situ hybridization to identify patients with prostate cancer previously exposed to XMRV infection and those who carry XMRV viral sequences in their prostate.METHODS Patients who had undergone radical prostatectomy were enrolled, and biologic specimens were obtained at surgery. The patients were genotyped for the R462Q RNASEL variant using a TaqMan genotyping assay on DNA from the peripheral blood. A serum assay that detects XMRV neutralizing antibodies was developed and used to determine which patients had serologic evidence of previous infection with XMRV virus. Some of these patients were also tested for the presence of XMRV nucleotide sequences in their prostate using polymerase chain reaction and fluorescence in situ hybridization analysis.RESULTS At a serum dilution of 1: 150, our assay detected 11 (27.5%) of 40 patients with XMRV neutralizing antibodies, including 8 (40%) of 20 with the RNASEL genotype QQ and 3 (15%) of 20 with either the RQ or RR genotype. These results were in complete concordance with 2 other assays ( polymerase chain reaction and fluorescence in situ hybridization), which were designed to detect XMRV infection.CONCLUSIONS XMRV infects some patients with prostate cancer. Neutralizing antibodies against XMRV correlated with 2 independent methods of detecting the virus in the prostate. The antibody response suggests that with clinical serologic assay development, it might be possible to screen patients for XMRV infection. The cases presented in the present report provided biologic samples that can be used for the development of a clinically relevant assay. UROLOGY 75: 755-761, 2010. Published by Elsevier Inc.