Leucine improves α-amylase secretion through the general secretory signaling pathway in pancreatic acinar cells of dairy calves
Leucine improves α-amylase secretion through the general secretory signaling pathway in pancreatic acinar cells of dairy calves
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亮氨酸通过一般分泌信号通路改善奶牛胰腺腺泡细胞的α-淀粉酶分泌
DOI：
10.1152/ajpcell.00396.2019
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发表时间：
2020-06-01
影响因子：
5.5
通讯作者：
Cao, Yangchun
中科院分区：
文献类型：
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作者：
Guo, Long;Tian, Huibin;Cao, Yangchun
本研究旨在通过对奶牛胰腺腺泡细胞（PA）蛋白质组学的研究，阐明亮氨酸影响胰腺酶尤其是淀粉酶分泌的机制。用四种浓度的亮氨酸（0、0.23、0.45和0.90 mM）处理实验模型PA细胞。检测四个亮氨酸处理组中不同蛋白质的丰度。无标记蛋白质组学分析能够在所有四个治疗组中鉴定出1，906种蛋白质，其中1，350种蛋白质在各组中显示出共同表达。补充亮氨酸的主要作用是增加（P < 0.05）柠檬酸合成酶和ATP酶活性，从而扩大胞浆ATP池，并上调分泌蛋白61（Sec61）的表达，从而促进蛋白分泌。总之，这些结果表明，亮氨酸增加TCA循环中的柠檬酸合酶和ATP酶活性，并促进Sec信号通路，以增加PA细胞的外分泌功能。
The present study aimed to elucidate the mechanisms by which leucine impacts the secretion of pancreatic enzymes, especially amylase, by studying the proteomics profiles of pancreatic acinar (PA) cells from dairy cows. PA cells, the experimental model, were treated with four concentrations of leucine (0, 0.23, 0.45, and 0.90 mM). The abundance of different proteins in the four leucine treatment groups was detected. Label-free proteomic analysis enabled the identification of 1,906 proteins in all four treatment groups, and 1,350 of these proteins showed common expression across the groups. The primary effects of leucine supplementation were increased (P < 0.05) citrate synthase and ATPase activity, which enlarged the cytosolic ATP pool, and the upregulation of secretory protein 61 (Sec61) expression, which promoted protein secretion. In summary, these results suggest that leucine increases citrate synthase in the TCA cycle and ATPase activity and promotes the Sec signaling pathway to increase the exocrine function of PA cells.