Physiological characterization of the Arabidopsis thaliana Oxidation-related Zinc Finger 1, a plasma membrane protein involved in oxidative stress
Physiological characterization of the Arabidopsis thaliana Oxidation-related Zinc Finger 1, a plasma membrane protein involved in oxidative stress
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DOI：
10.1007/s10265-010-0397-3
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发表时间：
2011-11-01
影响因子：
2.8
通讯作者：
Kim, Cheol Soo
中科院分区：
文献类型：
--
作者：
Huang, Ping;Chung, Moon-Soo;Kim, Cheol Soo
CCCH型锌指蛋白是一个含有串联锌结合基序的超家族，参与植物生长发育的许多方面。然而，这些蛋白质在植物胁迫耐受性中的确切作用知之甚少。本研究旨在探讨CCCH型锌指蛋白AtOZF 1（At 2g 19810）在氧化应激条件下的调节和功能作用。有趣的是，AtOZF 1蛋白定位于质膜。过氧化氢、脱落酸和盐度对AtOZF 1基因的转录有很强的诱导作用。AtOZF 1过表达的植物比野生型和T-DNA插入突变体atozf 1相对抗氧化胁迫。丙二醛，脂质过氧化的分解产物，积累在atozf 1突变体比野生型和AtOZF 1过表达的植物。此外，atozf 1突变体表现出较低的过氧化氢酶和愈创木酚过氧化物酶的活性，较高的失绿，和抗氧化基因的表达下调氧化胁迫。综上所述，这些观察结果表明，AtOZF 1所需的拟南芥氧化胁迫的耐受性。
The CCCH-type zinc finger proteins are a superfamily containing tandem zinc-binding motifs involved in many aspects of plant growth and development. However, the precise role of these proteins involved in plant stress tolerance is poorly understood. This study was to examine the regulatory and functional role of the CCCH-type zinc finger protein, AtOZF1 (At2g19810), under oxidative stress. Interestingly, the AtOZF1 protein was localized in the plasma membrane. The AtOZF1 transcripts were highly induced by treatment with hydrogen peroxide, abscisic acid and salinity. The AtOZF1-overexpressing plants were relatively resistant to oxidative stress than wild-type and T-DNA insertion mutant atozf1. Malondialdehyde, a decomposition product of lipid peroxidation, accumulated in atozf1 mutants more than in wild-type and AtOZF1-overexpressing plants. Furthermore, atozf1 mutants displayed lower activities of catalase and guaiacol peroxidase, higher chlorosis, and down-regulated expression of antioxidant genes under oxidative stress. Taken together, these observations demonstrate that AtOZF1 is required for the tolerance of Arabidopsis to oxidative stress.