ASSOCIATION KINETICS WITH COUPLED DIFFUSION .3. IONIC-STRENGTH DEPENDENCE OF LAC REPRESSOR-OPERATOR ASSOCIATION
ASSOCIATION KINETICS WITH COUPLED DIFFUSION .3. IONIC-STRENGTH DEPENDENCE OF LAC REPRESSOR-OPERATOR ASSOCIATION
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DOI：
10.1016/0301-4622(78)80010-6
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发表时间：
1978-01-01
影响因子：
3.8
通讯作者：
BLOMBERG, C
中科院分区：
文献类型：
--
作者：
BERG, OG;BLOMBERG, C
阻遏物-操纵子关联在模型中处理，其中阻遏物分子可以在大噬菌体λ上找到其特异性结合位点，即操纵子。 DNA 链通过沿着链执行一维扩散。离子强度依赖性的计算方法是在 DNA 链周围引入屏蔽静电势，并将该势中阻遏物的自由扩散与所提出的沿链的一维扩散耦合起来。对关联率的主要影响来自离子与非特异性 DNA 位点的竞争性结合。在阻遏物在全局意义上被束缚期间，沿链的扩散将由相当短距离内的严格一维运动组成，其间散布着许多局部解离，在此期间阻遏物本质上在溶液中是自由的。
The repressor-operator association is treated in a model where the repressor molecule can find its specific binding site, the operator, on a large phage .lambda. DNA chain by performing a 1-dimensional diffusion along the chain. The ionic-strength dependence is calculated by introducing a screened electrostatic potential around the DNA chain and coupling the free diffusion of the repressor in this potential to the proposed 1-dimensional diffusion along the chain. The main influence on the association rate comes from the competitive binding of ions to the unspecific DNA sites. During the time that the repressor is bound in a global sense, the diffusion along the chain will be made up of a strictly 1 dimensional motion over fairly short distances, interspersed with many local dissociations during which the repressor in essence is free in solution.