A large-scale phylogeny of Microhylidae inferred from a combined dataset of 121 genes and 427 taxa
A large-scale phylogeny of Microhylidae inferred from a combined dataset of 121 genes and 427 taxa
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从 121 个基因和 427 个类群的组合数据集中推断出的大规模 Microhylidae 系统发育
DOI：
10.1016/j.ympev.2018.03.036
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发表时间：
2018
影响因子：
4.1
通讯作者：
Zhang Peng
中科院分区：
文献类型：
--
作者：
Tu Na;Yang MengHua;Liang Dan;Zhang Peng
作为现存蛙类的第三大科，细蛙科的系统发育关系一直难以解决。在过去的十年中，几乎每个microhylid属都有大量的序列数据，但没有研究试图将这些数据联合收割机来重建该科的全面系统发育。在这项研究中，我们测定了20个接近完整或部分的线粒体基因组，并将它们与GenBank中所有可用的Microhylidae序列整合，构建了一个包含121个基因（14个线粒体和107个核蛋白编码基因）的超矩阵。组合的数据集是112，328字符长（每个物种的平均序列数据长度= 7829 bp），包括427 microhylid分类群，并涵盖了所有，但整个家庭的三个属。该数据集为11个名义亚科的传统分类提供了有力的支持，并提高了亚科之间关系的系统发育分辨率。非洲的Phrynomerinae亚科是所有其他microhylids的姐妹群，非洲的Hoplophryninae亚科是包括其余9个亚科的分支的姐妹分类单元。在属的水平上，我们的分析证实了大多数但不是所有的microhylid属的单系性。总之，我们提出了一个新的大规模的microhylid青蛙，应该是有价值的，解决他们的分类和比较进化研究的繁殖。
The phylogenetic relationships of Microhylidae, the third largest family of extant frogs, have been difficult to resolve. In the past decade, large amounts of sequence data have been deposited for almost every microhylid genus, but no study has attempted to combine these data to reconstruct a comprehensive phylogeny for this family. In this study, we sequenced 20 near-complete or partial microhylid mitochondrial genomes and integrated them with all available sequences of Microhylidae from GenBank to construct a supermatrix containing 121 genes (14 mitochondrial and 107 nuclear protein-coding genes). The combined dataset is 112,328 characters long (average sequence data length per species = 7829 bp), includes 427 microhylid taxa, and covers all but three genera of the entire family. This dataset provides strong support for the traditional classification of 11 nominal subfamilies and improves the phylogenetic resolution of the relationships among subfamilies. The African subfamily Phrynomerinae is the sister group of all the other microhylids, and the African subfamily Hoplophryninae is the sister taxon to a clade comprising the remaining 9 subfamilies. At the genus level, our analyses confirm the monophyly of most but not all microhylid genera. In summary, we present a new large-scale phylogeny of microhylid frogs that should be valuable for addressing their classification and for comparative evolutionary studies.