Multiresponsive and biocompatible self-healing hydrogel: its facile synthesis in water, characterization and properties
Multiresponsive and biocompatible self-healing hydrogel: its facile synthesis in water, characterization and properties
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多响应和生物相容性自愈水凝胶：其在水中的简便合成、表征和性能
DOI：
10.1039/c7sm00350a
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发表时间：
2017-04-28
期刊：
影响因子：
3.4
通讯作者：
Zhang, Qiqing
中科院分区：
文献类型：
--
作者：
Cheng, Cui;Zhang, Xiuli;Zhang, Qiqing
以支链普鲁兰多糖（A-Pul）、ε-聚赖氨酸（ε- PL）和支链聚乙烯亚胺（BPEI）为原料，通过Schiff碱反应制备了具有多响应性和生物相容性的自愈合ε- PL/A-Pul/BPEI水凝胶。亚胺键在80 s内迅速交联成水凝胶网络。扫描电子显微镜图像显示，水凝胶具有交联结构，平均孔径为58至82毫米。流变学测试表明，水凝胶保持良好的机械性能。水接触角和溶胀度研究表明，该水凝胶在水中能溶胀，最大溶胀率为1559%，pH和温度对平衡溶胀率有影响。水凝胶可以在凝胶化之前或之后注入，并且它们在室温下基于亚胺键的动态解偶联和再偶联在ddH（2）O中显示出自愈合过程。MTT实验表明，该水凝胶对小鼠骨髓间充质干细胞无细胞毒性。因此，该水凝胶在生物医学领域显示出潜在的应用，并因此进行了进一步的工作，使用自愈合水凝胶作为药物载体在体外/体内抗肿瘤研究。
Multiresponsive and biocompatible self-healing epsilon-PL/A-Pul/BPEI hydrogels were prepared in aqueous solution by Schiff base reaction with aldehyded pullulan (A-Pul), epsilon-poly-(l)-lysine (epsilon- PL) and branched polyethyleneimine (BPEI) as materials. The imine bonds were rapidly cross-linked into a hydrogel network within 80 s. Scanning electron microscopy images showed that the hydrogels exhibited a cross-linked structure with the average pore size from 58 to 82 mm. Rheology tests indicated that the hydrogels maintained good mechanical properties. Water contact angles and swelling studies suggested that the hydrogels could swell in water, with a max swell ratio of 1559%, and pH and temperature had an influence on the equilibrium swelling ratio. The hydrogels could be injected either before or after gelation, and they displayed a self-healing process in ddH(2)O at room temperature based on the dynamic uncoupling and recoupling of the imine bonds. The MTT assays implied that the hydrogels were non-cytotoxic on mice bone marrow mesenchymal stem cells. Therefore, the hydrogels showed potential application in biomedical fields, and consequently further work was performed using the self-healing hydrogels as drug carriers in in vitro/vivo antitumor studies.