Shape deformation and fission route of the lipid domain in a multicomponent vesicle.
Shape deformation and fission route of the lipid domain in a multicomponent vesicle.
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DOI：
10.1103/physreve.79.051924
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发表时间：
2009-05
期刊：
影响因子：
--
通讯作者：
Kai Yang;Xi Shao;Yuqiang Ma
中科院分区：
文献类型：
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作者：
Kai Yang;Xi Shao;Yuqiang Ma
本文用耗散粒子动力学方法研究了多组分囊泡中脂微区的曲率变化和裂变路径。在不同的脂类分布和不同的囊泡面积体积比条件下，我们得到了不同的结构域在囊泡中的构型：观察到了脂域的三种典型的曲率特征，即相对于囊泡的正曲率、负曲率和恒曲率。此外，在我们的模拟中还获得了结构域的其他一些形态和两种囊泡分裂途径(即胞外和内胞分裂)。特别强调了负曲率域的形成和内吞分裂的行为。基于我们的模拟，表明水在囊泡中结构域的内陷和内吞分裂过程中起着重要作用。对于胞内分裂，在水的作用下，不同大小的结构域根据不同的路径进化。此外，我们还发现，脂质分子的自发曲率和面积体积比都可以促进或抑制脂质结构域的形状变形。在这两种因素的竞争下，在我们的模拟中观察到了另一种可能的内吞分裂途径，即只有一部分脂域内陷到囊泡内部完成内吞分裂。我们的研究有助于了解细胞膜形状变化的可能机制以及几种囊泡分裂途径的差异。
In this paper, the curvature changes and fission routes of the lipid domains in a multicomponent vesicle are studied by dissipative particle dynamics. Under different conditions of asymmetric distribution of lipids in two leaflets of lipid bilayer and area-to-volume ratio of the vesicle, we obtained different configurations of the domain in the vesicle: three typical curvature characters of the lipid domain, namely, positive, negative, and invariable curvatures compared to the vesicle are observed. Furthermore, some other morphologies of the domain and two vesicle fission routes (i.e., exocytic and endocytic fissions) are also obtained in our simulations. Particular emphasis is put on the formation of the negative curvature domain and on the behavior of endocytic fission. Based on our simulations, it is indicated that water plays an important role in the invagination and endocytic fission processes of the domain in a vesicle. For endocytic fission, domains of different sizes are evolved according to different routes under the effect of the water. Additionally, we find that both the spontaneous curvature of lipid molecules and area-to-volume ratio can promote or restrain the shape deformation of the lipid domain. Under the competition of these two factors, another possible route of endocytic fission is observed in our simulations, in that only a part of the lipid domain invaginates into the interior of the vesicle to complete the endocytic fission. Our study is helpful for understanding the possible mechanism of the shape transformation of the cellular membrane and the difference of several kinds of routes of vesicle fission.