Facile and rapid detection of respiratory syncytial virus using metallic nanoparticles.
Facile and rapid detection of respiratory syncytial virus using metallic nanoparticles.
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DOI：
10.1186/s12951-016-0167-z
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发表时间：
2016-02-27
影响因子：
10.2
通讯作者：
Singh SR
中科院分区：
文献类型：
--
作者：
Valdez J;Bawage S;Gomez I;Singh SR
呼吸道合胞病毒（RSV）在婴儿、儿童和老年人中引起严重的呼吸道感染。目前，没有有效的疫苗或治疗RSV的特异性药物。然而，抗病毒药物利巴韦林和帕利珠单抗是与对症治疗一起开的。RSV检测对于确保儿童得到适当治疗非常重要。RSV最常用的检测方法是DFA、ELISA和Real-time PCR，这些方法昂贵且耗时。近年来，利用金属纳米材料的局部表面等离子体共振（LSPR）光谱等等离子体探测技术得到了人们的广泛关注。与现有的表面增强拉曼散射（SERS）和质谱等生物物理检测技术相比，LSPR光谱技术简单易行。在本研究中，我们利用LSPR移位作为RSV检测方法，利用抗RSV多克隆抗体偶联金属纳米颗粒（Cu， Ag和Au）。以海藻酸盐为还原剂和稳定剂合成纳米颗粒。测量了所有三种金属纳米颗粒（非功能化和功能化）的RSV剂量和时间依赖性LSPR位移。在铜绿假单胞菌和腺病毒存在的情况下，评估了功能化纳米颗粒对RSV的特异性。我们发现功能化铜纳米颗粒在RSV检测中是有效的。功能化铜纳米粒子和银纳米粒子分别在腺病毒和铜绿假单胞菌存在下对RSV具有特异性。检出限和定量限表明，功能化铜纳米粒子优于银纳米粒子和金纳米粒子。该研究证明了LSPR在RSV检测中的成功应用，为基于LSPR的检测设备的潜在开发提供了一种简单而廉价的替代方法。本文的在线版本（doi:10.1186/s12951-016-0167-z）包含补充材料，可供授权用户使用。
Respiratory syncytial virus (RSV) causes severe respiratory infection in infants, children and elderly. Currently, there is no effective vaccine or RSV specific drug for the treatment. However, an antiviral drug ribavirin and palivizumab is prescribed along with symptomatic treatment. RSV detection is important to ensure appropriate treatment of children. Most commonly used detection methods for RSV are DFA, ELISA and Real-time PCR which are expensive and time consuming. Newer approach of plasmonic detection techniques like localized surface plasmon resonance (LSPR) spectroscopy using metallic nanomaterials has gained interest recently. The LSPR spectroscopy is simple and easy than the current biophysical detection techniques like surface-enhanced Raman scattering (SERS) and mass-spectroscopy. In this study, we utilized LSPR shifting as an RSV detection method by using an anti-RSV polyclonal antibody conjugated to metallic nanoparticles (Cu, Ag and Au). Nanoparticles were synthesized using alginate as a reducing and stabilizing agent. RSV dose and time dependent LSPR shifting was measured for all three metallic nanoparticles (non-functionalized and functionalized). Specificity of the functionalized nanoparticles for RSV was evaluated in the presence Pseudomonas aeruginosa and adenovirus. We found that functionalized copper nanoparticles were efficient in RSV detection. Functionalized copper and silver nanoparticles were specific for RSV, when tested in the presence of adenovirus and P. aeruginosa, respectively. Limit of detection and limit of quantification values reveal that functionalized copper nanoparticles are superior in comparison with silver and gold nanoparticles. The study demonstrates successful application of LSPR for RSV detection, and it provides an easy and inexpensive alternative method for the potential development of LSPR-based detection devices. The online version of this article (doi:10.1186/s12951-016-0167-z) contains supplementary material, which is available to authorized users.