Anaerobes and methanogens dominate the microbial communities in water harvesting ponds used by Kenyan rural smallholder farmers.
Anaerobes and methanogens dominate the microbial communities in water harvesting ponds used by Kenyan rural smallholder farmers.
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DOI：
10.1016/j.scitotenv.2022.153040
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发表时间：
2022-01
期刊：
影响因子：
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通讯作者：
Benjamin H Gregson;A. Bani;Laurel A. Steinfield;D. Holt;C. Whitby
中科院分区：
文献类型：
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作者：
Benjamin H Gregson;A. Bani;Laurel A. Steinfield;D. Holt;C. Whitby
肯尼亚许多农村小农户使用集水池收集雨水，作为家庭和农业用水的可持续来源。目前关于这些池塘中微生物生态的信息有限。在这里，我们使用高通量测序（HTS）来表征存在的微生物（包括潜在的病原体和指示物种），以及离子色谱法来测量水化学（阴离子和阳离子浓度）。氟和镁浓度是微生物群落最强的预测变量。专性或兼性厌氧细菌属（如SpirochaetaandOpitutus）是丰富的细菌群落中，而Woesearchaeota和产甲烷菌占主导地位的古菌群落。这表明池塘中的水是缺氧或缺氧的，如果用于灌溉，可能会影响作物产量和生存能力。此外，还发现了机会致病菌非结核分枝杆菌（NTM），即偶然分枝杆菌，占细菌群落的>1%，这表明存在潜在的人类健康风险。在这里，我们建议低成本的改变池塘管理，以改善或改善池塘缺氧和消除病原体，以造福于这些农场的生计和福利。这项研究还表明，HTS广泛筛选微生物群落，评估水质，并确定潜在的致病群体的适用性。
Many rural smallholder farmers in Kenya use water-harvesting ponds, to collect rainwater, as sustainable sources of water for domestic and agricultural purposes. There is currently limited information regarding the microbial ecology in these ponds. Here, we used High Throughput Sequencing (HTS) to characterize the microorganisms present (including potential pathogens and indicator species) alongside ion chromatography to measure water chemistry (anion and cation concentration). Fluoride and magnesium concentration were the strongest predictor variables of the microbial community. Obligately or facultatively anaerobic bacterial genera (e.g.SpirochaetaandOpitutus) were abundant within the bacterial community, whilst Woesearchaeota and methanogens dominated the archaeal community. This suggests the water in the ponds is hypoxic or anoxic, and if used for irrigation, may potentially impact crop yield and viability. In addition, the opportunistic pathogen non-tuberculous mycobacteria (NTM),Mycobacterium fortuitumwas found, comprising >1% of the bacterial community, suggesting a potential human health risk. Here we suggest low-cost changes to pond management, to improve or ameliorate pond anoxia and remove pathogens to benefit the livelihoods and welfare of these farms. This study also shows the applicability of HTS to broadly screen the microbial communities, assess water quality, and identify potentially pathogenic groups.