ELECTRICAL-STIMULATION AND RECORDING FROM CULTURED NEURONS USING A PLANAR ELECTRODE ARRAY
ELECTRICAL-STIMULATION AND RECORDING FROM CULTURED NEURONS USING A PLANAR ELECTRODE ARRAY
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DOI：
10.1016/0302-4598(92)80067-q
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发表时间：
1992-12-01
期刊：
影响因子：
--
通讯作者：
KAWANA, A
中科院分区：
文献类型：
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作者：
JIMBO, Y;KAWANA, A
采用现代半导体技术制备了平面电极阵列。成功地在氧化铟锡电极图案表面培养了小鼠和鸡背根神经节细胞。通过利用培养基质的表面特性来引导神经突生长，建立了神经突与基质电极之间的接触。引导神经突用电刺激基底电极，并记录细胞反应。细胞内和细胞外。可以观察到细胞膜的去极化以及响应刺激的动作电位的产生。结果表明，这种平面电极阵列可以成为一种有用的非侵入性电生理测量工具。
Planar electrode arrays were fabricated using modern semiconductor technology. Mouse and chick dorsal root ganglion cells were successfully cultured on the surface of indium tin oxide electrode patterns. Contact between the neurites and the substrate electrodes was established by exploiting the surface properties of the culture substrates to guide neurite outgrowth. The guided neurites were stimulated electrically using the substrate electrodes and cell responses were recorded. intracellularly and extracellularly. Depolarization of the cell membrane as well as generation of action potentials in response to the stimulation could be observed. The results suggest that this type of planar electrode array could be a useful tool for non-invasive electrophysiological measurements.