A gluten metabolism study in healthy individuals shows the presence of faecal glutenasic activity
A gluten metabolism study in healthy individuals shows the presence of faecal glutenasic activity
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DOI：
10.1007/s00394-011-0214-3
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发表时间：
2012-04-01
影响因子：
5
通讯作者：
Casqueiro, Javier
中科院分区：
文献类型：
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作者：
Caminero, Alberto;Nistal, Esther;Casqueiro, Javier
为研究健康人谷蛋白代谢及其对肠道微生物活性的影响，对11名健康人在4种饮食方案下的粪便进行了分析：正常谷蛋白饮食、严格无谷蛋白饮食（GFD）、GFD加9 g谷蛋白/d和GFD加30 g谷蛋白/d。结果表明，粪中谷蛋白含量、粪胰蛋白酶活性（FTA）、短链脂肪酸（SCFAs）和粪谷蛋白酶活性（FGA）随日粮中谷蛋白摄入量的不同而有显著差异。当在饮食中施用高面筋剂量（30 g/天）时，SCFA浓度（70.5 mmol/kg粪便）与实验的GFD期间的SCFA浓度（33.8 mmol/kg粪便）显著不同。然而，FTA显示GFD（34单位）和正常含麸质饮食（60单位）之间以及GFD和GFD + 30 g麸质/天（67单位）之间存在显著差异。当麸质存在于饮食中时，在粪便中检测到麸质，表明摄入的至少一部分麸质在大肠中被消除，为肠道微生物蛋白酶提供底物。我们还显示了粪便谷蛋白酶活性的存在，其与饮食中的谷蛋白摄入量成比例地增加，显示DSG中的酶活性为993单位，DSG + 9 g中的酶活性为2，063单位，DSG + 30 g中的酶活性为6，090单位。肠道微生物群的活性通过饮食中的谷蛋白摄入量而改变。在饮食中掺入谷蛋白增加了粪便中谷蛋白蛋白蛋白水解活性的活性。
To study the gluten metabolism in healthy individuals and its effect over the intestinal microbial activity.The faeces of eleven healthy subjects were analysed under 4 diet regimens: their normal gluten diet, a strict gluten-free diet (GFD), a GFD with a supplemental intake of 9 g gluten/day and a GFD with a supplemental intake of 30 g gluten/day. Gluten content, faecal tryptic activity (FTA), short-chain fatty acids (SCFAs) and faecal glutenasic activity (FGA) were analysed in faecal samples.Faecal gluten contents, FTA, SCFAs and FGA varied significantly with different levels of gluten intake in the diet. When high gluten doses (30 g/day) were administered in the diet, SCFA concentrations (70.5 mmoles/kg faeces) were significantly different from those from the GFD period (33.8 mmoles/kg faeces) of the experiment. However, the FTA showed significant differences between the GFD (34 units) and the normal gluten-containing diet (60 units) and also between the GFD and the GFD + 30 g of gluten/day (67 units). When gluten was present in the diet, gluten was detected in the faeces, showing that at least a portion of the gluten ingested is eliminated in the large intestine, providing a substrate for intestinal microbial proteases. We have also shown the presence of faecal glutenasic activity that increased proportionally with the gluten intake in the diet, showing an enzymatic activity of 993 units in DSG, 2,063 units in DSG + 9 g and 6,090 units in DSG + 30 g.The activity of the intestinal microbiota is modified by gluten intake in the diet. The incorporation of gluten in the diet increases the activity of a gluten proteolytic activity in the faeces.