A LACTOCOCCAL EXPRESSION SYSTEM FOR ENGINEERED NISINS
A LACTOCOCCAL EXPRESSION SYSTEM FOR ENGINEERED NISINS
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DOI：
10.1128/aem.58.11.3683-3693.1992
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发表时间：
1992-11-01
影响因子：
4.4
通讯作者：
GASSON, MJ
中科院分区：
文献类型：
--
作者：
DODD, HM;HORN, N;GASSON, MJ
乳酸乳球菌（Lactococcus lacactis）菌株FI5876已被改造并开发用于工程乳酸乳球菌（nisin）变体的表达系统。驻留的nisA基因的插入失活对下游基因有极性影响，包括那些与nisin免疫有关的基因。然而，随后该区域的染色体重排涉及新发现的插入元件（IS905），产生了缺乏nisA基因产物的菌株，但表达了前泌素成熟、分泌和免疫所必需的nisin决定因子。含有位点特异性突变的质粒编码的nisA基因对nisA基因的损伤进行互补，导致只产生含有特定氨基酸取代的改变的nisins。
The nisin-producing Lactococcus lactis strain FI5876 has been modified and developed for use as an expression system for engineered nisin variants. Insertional inactivation of the resident nisA gene had a polar effect on downstream genes, including those involved in nisin immunity. However, subsequent chromosomal rearrangements in this region involving a newly discovered insertion element (IS905) generated a strain that was deficient in the nisA gene product but expressed those nisin determinants necessary for prenisin maturation, secretion, and immunity. Complementation of the lesion in the nisA gene by plasmid-encoded nisA genes containing site-specific mutations resulted in the exclusive production of altered nisins containing specific amino acid substitutions.