Anti-inflammatory and analgesic activities of mature fresh leaves of Vitex negundo
Anti-inflammatory and analgesic activities of mature fresh leaves of Vitex negundo
复制标题
DOI：
10.1016/s0378-8741(03)00159-4
复制
发表时间：
2003-08-01
影响因子：
5.4
通讯作者：
Ratnasooriya, WD
中科院分区：
文献类型：
--
作者：
Dharmasiri, MG;Jayakody, JRAC;Ratnasooriya, WD
本研究通过给大鼠口服该叶子的水提取物，证实了阿育吠陀医学中声称的 Vitex negundo L.（马鞭草科）成熟新鲜叶子 (MFL) 的口服抗炎、镇痛和抗组胺特性。卡拉胶诱导的大鼠爪水肿的早期阶段（2小时）被MFL显着抑制（P < 0.01），呈反剂量依赖性（r(2) = 1，P < 0.01）。 EC50 为 2 g/kg MFL。在甲醛引起的大鼠爪水肿试验中，2.5 和 5 g/kg 的叶子在试验第 4-6 天显着（P < 0.05）抑制炎症。在热板试验中，2.5 和 5 g/kg 的 MFL 在治疗 1 小时时表现出显着（P < 0.05）且直接剂量依赖性的镇痛活性，而在大鼠甩尾试验中则没有这种活性。镇痛活性的 EC50 为 4.1 e/kg。在福尔马林试验中，1.25、2.5和5 g/kg的MFL与阿司匹林一样，在试验的两个阶段均显着（P < 0.05）抑制疼痛。叶子表现出反剂量依赖性的体内抗组胺药和体外前列腺素（PG）合成抑制、膜稳定和抗氧化活性。纳洛酮在热板试验中并未消除镇痛活性。 5 g/kg 的 MFL 不会损害肌肉力量和协调性，也不会引起镇静。 5 g/kg MFL 治疗没有显示出急性毒性或应激迹象。口服 5 g/kg MFL 治疗 14 天后，血清 AST 活性显着增加。树的开花并没有消除叶子的镇痛和抗炎活性。这些观察结果表明，荆条的新鲜叶具有抗炎和镇痛活性，可能是通过抑制 PG 合成、抗组胺、膜稳定和抗氧化活性介导的。抗组胺活性可以产生阿育吠陀医学中声称的止痒作用。 (C) 2003 年，爱思唯尔科学爱尔兰有限公司出版。
This study confirmed the oral anti-inflammatory, analgesic and antihistamine properties of mature fresh leaves (MFL) of Vitex negundo L. (Verbenaceae) claimed in the Ayurveda medicine by orally treating a water extract of the leaves to rats. The early phase (2 h) of carrageenan-induced rat paw oedema was significantly (P < 0.01) suppressed in an inversely does-dependent (r(2) = 1, P < 0.01) manner by MFL. The EC50 was 2 g/kg of MFL. In the formaldehyde-induced rat paw oedema test, the 2.5 and 5 g/kg leaves significantly (P < 0.05) suppressed the inflammation on days 4-6 of the test. In the hot plate test, 2.5 and 5 g/kg of MFL showed a significant (P < 0.05) and directly dose-dependent analgesic activity at I h of treatment while the activity was absent in the tail flick test in rats. The EC50 for the analgesic activity was 4.1 e/kg. In the formalin test, 1.25, 2.5 and 5 g/kg of MFL significantly (P < 0.05) suppressed the pain in both the phases of the test like aspirin. The leaves showed an inversely dose-dependent in vivo antihistamine and in vitro prostaglandin (PG) synthesis inhibition, membrane stabilising and antioxidant activities. Naloxone did not abolish the analgesic activity in the hot plate test. A 5 g/kg of MFL did not impair muscle strength and co-ordination and did not induce sedation. The treatment of 5 g/kg of MFL did not show signs of acute toxicity or stress. Fourteen-day oral treatment of 5 g/kg of MFL significantly increased the serum activity of AST. Flowering of the tree did not abolish the analgesic and anti-inflammatory activities of the leaves. These observations revealed that the fresh leaves of Vitex negundo have anti-inflammatory and pain suppressing activities possibly mediated via PG synthesis inhibition, antihistamine, membrane stabilising and antioxidant activities. The antihistamine activity can produce the anti-itching effect claimed in Ayurveda medicine. (C) 2003 Published by Elsevier Science Ireland Ltd.