Antimicrobial synergistic effect of linolenic acid and monoglyceride against Bacillus cereus and Staphylococcus aureus
Antimicrobial synergistic effect of linolenic acid and monoglyceride against Bacillus cereus and Staphylococcus aureus
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DOI：
10.1021/jf011175a
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发表时间：
2002-03-27
影响因子：
6.1
通讯作者：
Shin, DH
中科院分区：
文献类型：
--
作者：
Lee, JY;Kim, YS;Shin, DH
在肉汤培养基中测定了亚麻酸与单甘油酯（月桂酸甘油酯或肉豆蔻酸甘油酯）对6种食源性微生物的抗菌作用。亚麻酸对蜡状芽孢杆菌和金黄色葡萄球菌的最低抑菌浓度分别为20和50 ppm。B的生长。用10 ppm的亚麻酸和10 ppm的单酸甘油酯处理的蜡状芽孢杆菌比单独的亚麻酸更具有抑制作用，并且与对照相比，活细胞群体减少2-4个对数周期。当亚麻酸以该水平添加时，与对照相比，细胞外液的三磷酸腺苷（ATP）浓度急剧增加，并且与单甘油酯的组合效果高于单独使用亚麻酸的效果。但细胞内ATP浓度较对照组降低。从这些结果，我们得出结论，亚麻酸对B具有很强的抗菌活性。cereus和S.金黄色葡萄球菌，并且亚麻酸与单甘油酯组合显示出比单独使用亚麻酸更强的抗微生物活性。
The antimicrobial effect of linolenic acid with or without monoglyceride (glycerol laurate or glycerol myristate) against six food-borne microorganisms was determined in broth medium. Minimum inhibitory concentration of linolenic acid on Bacillus cereus and Staphylococcus aureus was 20 and 50 ppm, respectively. The growth of B. cereus treated with linolenic acid at 10 ppm with 10 ppm monoglyceride was more inhibitory than that of linolenic acid alone, and the viable cell population was reduced 2-4 log cycles compared to that of the control. When linolenic acid was added at that level, the adenosine triphosphate (ATP) concentration of extracellular fluid was drastically increased compared with that of the control, and the combined effect with monoglyceride was higher than that with linolenic acid alone. However, the intracellular ATP concentration decreased compared with that of the control. From these results, we concluded that linolenic acid has a strong antimicrobial activity against B. cereus and S. aureus, and that linolenic acid combined with monoglyceride showed stronger antimicrobial activity than using linolenic acid alone.