MICE IMMUNIZED WITH A SUBVIRAL PARTICLE CONTAINING THE JAPANESE ENCEPHALITIS-VIRUS PRM/M AND E-PROTEINS ARE PROTECTED FROM LETHAL JEV INFECTION
MICE IMMUNIZED WITH A SUBVIRAL PARTICLE CONTAINING THE JAPANESE ENCEPHALITIS-VIRUS PRM/M AND E-PROTEINS ARE PROTECTED FROM LETHAL JEV INFECTION
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DOI：
10.1016/0042-6822(92)90526-u
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发表时间：
1992-06-01
期刊：
影响因子：
3.7
通讯作者：
MASON, PW
中科院分区：
文献类型：
--
作者：
KONISHI, E;PINCUS, S;MASON, PW
用编码日本脑炎病毒（JEV）prM和E基因的重组痘苗病毒感染HeLa细胞，获得细胞外亚病毒颗粒。这些颗粒含有包埋在脂质双层中的JEV prM/M和E蛋白，并且使用聚合酶链反应和识别JEV E基因的一部分的引物在颗粒中未检测到RNA。该颗粒是均匀的球形，直径为20 nm，在其表面上具有5 nm的突起。用纯化的细胞外颗粒与弗氏完全佐剂乳化后，单次接种小鼠，对4.9 × 105 LD_（50）的JEV具有完全的保护作用。中和和血凝抑制抗体滴度和放射免疫沉淀数据的比较表明，免疫接种的颗粒诱导的免疫应答类似于接种重组牛痘病毒。
Extracellular subviral particles produced by HeLa cells infected with a recombinant vaccinia virus encoding the prM and E genes of Japanese encephalitis virus (JEV) were purified and characterized. These particles contained the JEV prM/M and E proteins embedded in a lipid bilayer, and RNA was not detected in particles using the polymerase chain reaction and primers recognizing a part of the JEV E gene. The particles were uniformly spherical with a 20-nm diameter and had 5-nm projections on their surface. Mice that received a single inoculation of the purified extracellular particles emulsified with Freund's complete adjuvant were fully protected against 4.9 × 105LD50of JEV. Comparison of the neutralizing and hemagglutination-inhibiting antibody titers and radioimmunoprecipitation data showed that immunization with the particles induced an immune response similar to that following inoculation with the recombinant vaccinia virus.