Structure and topology of microbial communities in the major gut compartments of Melolontha melolontha larvae (Coleoptera: Scarabaeidae)
Structure and topology of microbial communities in the major gut compartments of Melolontha melolontha larvae (Coleoptera: Scarabaeidae)
复制标题
DOI：
10.1128/aem.71.8.4556-4566.2005
复制
发表时间：
2005-08-01
影响因子：
4.4
通讯作者：
Friedrich, MW
中科院分区：
文献类型：
--
作者：
Egert, M;Stingl, U;Friedrich, MW
研究了欧洲cock虫（Melolontha Melolontha）的肠道肠道幼虫的主要肠道室中肠道菌群的物理化学肠道条件以及肠道菌群的组成和拓扑结构。用微传感器测量pH，0，H和氧化还原电位的轴向和径向谱。在单个幼虫的中肠样品中细菌16S rRNA基因的末端限制片段长度多态性（T-RFLP）分析显示出一种简单但可能是非特异性的社区结构。相比之下，后肠样品的T-RFLP轮廓更加多样化，但高度相似，尤其是在墙部馏分中，表明存在参与消化的肠道特异性群落。虽然中肠和后肠（34和15 mm）中的高醋酸浓度证实了两个隔室中微生物发酵的存在，但甲烷生成仅限于后肠。甲烷摩托杆菌属。是唯一检测到的甲烷元素，仅限于该隔室。后肠的细菌16S rRNA基因克隆文库以与梭子座有关的克隆为主。与静脉细菌，芽孢杆菌，乳酸菌和伽马杆菌相关的克隆仅限于管腔，而与β-甲状腺细菌相关的克隆仅在后肠壁上发现。基于PCR的分析和与组特异性寡核苷酸探针的全细胞的原位杂交的结果表明，脱硫相关细菌占后肠壁细菌群落的10％至15％。从欧洲其他四个人群的幼虫中硫酸盐特异性腺苷-5'-磷酸硫酸酯还原酶基因APSA限制到后肠壁的DNA提取物的限制表明，硫酸盐还原物通常会在这种生境中定居。
Physicochemical gut conditions and the composition and topology of the intestinal microbiota in the major gut compartments of the root-feeding larva of the European cockchafer (Melolontha melolontha) were studied. Axial and radial profiles of pH, 0, H, and redox potential were measured with microsensors. Terminal restriction fragment length polymorphism (T-RFLP) analysis of bacterial 16S rRNA genes in midgut samples of individual larvae revealed a simple but variable and probably nonspecific community structure. In contrast, the T-RFLP profiles of the hindgut samples were more diverse but highly similar, especially in the wall fraction, indicating the presence of a gut-specific community involved in digestion. While high acetate concentrations in the midgut and hindgut (34 and 15 mM) corroborated the presence of microbial fermentation in both compartments, methanogenesis was confined to the hindgut. Methanobrevibacter spp. were the only methanogens detected and were restricted to this compartment. Bacterial 16S rRNA gene clone libraries of the hindgut were dominated by clones related to the Clostridiales. Clones related to the Actinobacteria, Bacillales, Lactobacillales, and gamma-Proteobacteria were restricted to the lumen, whereas clones related to the beta- and delta-Proteobacteria were found only on the hindgut wall. Results of PCR-based analyses and fluorescence in situ hybridization of whole cells with group-specific oligonucleotide probes documented that Desulfovibrio-related bacteria comprise 10 to 15% of the bacterial community at the hindgut wall. The restriction of the sulfate-reducer-specific adenosine-5'-phosphosulfate reductase gene apsA to DNA extracts of the hindgut wall in larvae from four other populations in Europe suggested that sulfate reducers generally colonize this habitat.