The Putative Polymerase Sequence of Infectious Salmon Anemia Virus Suggests a New Genus within theOrthomyxoviridae
The Putative Polymerase Sequence of Infectious Salmon Anemia Virus Suggests a New Genus within theOrthomyxoviridae
复制标题
传染性鲑鱼贫血病毒的推定聚合酶序列表明正粘病毒科中存在一个新属
作者：
B. Krossøy;I. Hordvik;F. Nilsen;A. Nylund;C. Endresen
摘要传染性鲑鱼贫血病毒（ISAV）是一种感染硬骨鱼类的正粘病毒样病毒。这种病毒引起的疾病对挪威、加拿大和苏格兰的大西洋鲑鱼养殖业造成了重大的经济后果。在这项工作中，我们报告了一个ISAV特异性cDNA的克隆和测序，该cDNA包含2,245 bp，具有一个开放阅读框架，编码一个预测的蛋白质，计算分子量为80.5 kDa。推定的蛋白质序列显示了所有病毒RNA依赖性RNA聚合酶的核心聚合酶基序特征。与其他节段负链RNA病毒的相应区域的保守基序的比较显示出与正粘病毒科的成员比与其他家族的病毒更密切的关系。推定的ISAV聚合酶蛋白（PB 1）具有708个氨基酸的长度，在中性pH下电荷为+22，pI为9.9，这与该家族其他成员的PB 1蛋白的性质一致。不同PB 1蛋白之间的距离的计算表明，ISAV与该家族的其他成员关系较远，但与流感病毒的关系比与托戈托病毒的关系更密切。基于这些和以前发表的结果，我们建议ISAV包括一个新的，第五属的正粘病毒科。
ABSTRACT The infectious salmon anemia virus (ISAV) is an orthomyxovirus-like virus infecting teleosts. The disease caused by this virus has had major economic consequences for the Atlantic salmon farming industry in Norway, Canada, and Scotland. In this work, we report the cloning and sequencing of an ISAV-specific cDNA comprising 2,245 bp with an open reading frame coding for a predicted protein with a calculated molecular weight of 80.5 kDa. The putative protein sequence shows the core polymerase motifs characteristic of all viral RNA-dependent RNA polymerases. Comparison of the conserved motifs with the corresponding regions of other segmented negative-stranded RNA viruses shows a closer relationship with members of the Orthomyxoviridae than with viruses in other families. The putative ISAV polymerase protein (PB1) has a length of 708 amino acids, a charge of +22 at neutral pH, and a pI of 9.9, which are consistent with the properties of the PB1 proteins of other members of the family. Calculations of the distances between the different PB1 proteins indicate that the ISAV is distantly related to the other members of the family but more closely related to the influenza viruses than to the Thogoto viruses. Based on these and previously published results, we propose that the ISAV comprises a new, fifth genus in the Orthomyxoviridae.
加帽异源 RNA 引发流感病毒 RNA 转录。
影响因子：
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作者：
Krug,RM
通讯作者：
Krug,RM