Templated chemistry for bioorganic synthesis and chemical biology
Templated chemistry for bioorganic synthesis and chemical biology
复制标题
DOI：
10.1002/psc.3198
复制
发表时间：
2019-07-01
影响因子：
2.1
通讯作者：
Seitz, Oliver
中科院分区：
文献类型：
--
作者：
Seitz, Oliver
鉴于 2018 年 Max Bergmann 奖章，这篇综述讨论了作者实验室开发的化学生物学驱动的模板化学的进展。重点综述介绍了用于在空间中定向功能单元（例如功能基团、发色团、生物分子或配体）的模板类别。蛋白质合成中应用的单分子模板有利于未受保护的肽的片段偶联。通过双分子组装体进行模板化可以控制两个分子功能单元之间的邻近关系。作为一个有启发性的例子，将展示活细胞上受体蛋白的卷曲螺旋肽模板标记。术语分子组装体提供了将两个（生物）分子上功能单元的邻近性置于第三方分子控制下的机会。这使得可以设计条件双分子反应。一个值得注意的例子是 DNA/RNA 触发的肽合成。最后一部分展示了如何使用术语分子和多分子组装体来更好地表征和理解多价蛋白质-配体相互作用。
In light of the 2018 Max Bergmann Medal, this review discusses advancements on chemical biology-driven templated chemistry developed in the author's laboratories. The focused review introduces the template categories applied to orient functional units such as functional groups, chromophores, biomolecules, or ligands in space. Unimolecular templates applied in protein synthesis facilitate fragment coupling of unprotected peptides. Templating via bimolecular assemblies provides control over proximity relationships between functional units of two molecules. As an instructive example, the coiled coil peptide-templated labelling of receptor proteins on live cells will be shown. Termolecular assemblies provide the opportunity to put the proximity of functional units on two (bio)molecules under the control of a third party molecule. This allows the design of conditional bimolecular reactions. A notable example is DNA/RNA-triggered peptide synthesis. The last section shows how termolecular and multimolecular assemblies can be used to better characterize and understand multivalent protein-ligand interactions.