Isolation of herpes simplex virus nucleocapsid DNA.
Isolation of herpes simplex virus nucleocapsid DNA.
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单纯疱疹病毒核衣壳 DNA 的分离。
DOI：
10.1007/978-1-4939-0428-0_3
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Szpara,Moriah
中科院分区：
文献类型：
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作者：
Szpara,Moriah
作为一种无生命的病毒，单纯疱疹病毒1型（HSV-1）必然在其DNA中编码其所有功能。纯病毒DNA的分离允许多种下游应用，包括重组HSV株的产生、选定区域的克隆和病毒DNA的测序。术语核衣壳是指病毒基因组与封闭衣壳的组合;这些病毒基因组必须是线性的，并且已经被包装用于外出，即使它们尚未从细胞中释放。相比之下，不与衣壳相关的病毒DNA可以包括附加体或串联形式，并且可以具有修饰，例如在细胞内添加的组蛋白。在该方案中，病毒衣壳保护HSV基因组免受剥离和破坏大多数细胞污染物的试剂的影响。该程序描述了病毒核衣壳的分离及其随后的溶解以纯化干净的线性HSV DNA。
As an inanimate virus, herpes simplex virus type 1 (HSV-1) necessarily encodes all of its functions in its DNA. Isolation of pure viral DNA allows multiple downstream applications, including the creation of recombinant HSV strains, cloning of selected regions, and sequencing of viral DNA. The term nucleocapsid refers to the combination of the viral genome with the enclosing capsid; these viral genomes are necessarily linear and have been packaged for egress, even if they are not yet released from the cell. In contrast, viral DNA that is not associated with capsids may include episomal or concatenated forms and may have modifications such as histones that are added within cells. During this protocol, the viral capsid protects the HSV genome from reagents that strip away and destroy most cellular contaminants. This procedure describes the isolation of viral nucleocapsids and their subsequent dissolution to purify clean, linear HSV DNA.