Comparison of the antigens produced by foamy virus in a cytolytic and a persistent infection of HEp2 cells.
Comparison of the antigens produced by foamy virus in a cytolytic and a persistent infection of HEp2 cells.
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DOI：
10.1099/0022-1317-44-1-235
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发表时间：
1979-07
期刊：
影响因子：
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通讯作者：
E. Gould;J. Hartley
中科院分区：
文献类型：
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作者：
E. Gould;J. Hartley
当用同源超免疫兔抗血清进行免疫扩散测试时，来自 I 型猿泡沫病毒对 HEp2 细胞的溶细胞感染的抗原产生两条多组分沉淀线。从HEp2细胞中MK5病毒的非生产性感染获得的抗原仅产生那些与从溶细胞感染获得的内线相对应的沉淀线。类似地，针对从持续感染中提取的抗原的超免疫兔抗血清缺乏导致外线沉淀的抗体。使用同源和异源血清对丙酮固定和未固定的细胞进行间接免疫荧光证实了持续感染中不存在抗原，并表明在持续感染的细胞中产生了抗原，该抗原在溶细胞感染的细胞中不存在或存在非常少量。中和实验和乙醚处理表明，持续感染中缺失的抗原是泡沫病毒的包膜成分。有人提出，持续感染与一些 RNA 肿瘤病毒感染具有共同的特性。
The antigens from cytolytic infections of HEp2 cells by type I simian foamy virus produced two multicomponent precipitation lines when tested by immunodiffusion with the homologous hyperimmune rabbit antiserum. The antigens obtained from a non-productive infection of MK5 virus in HEp2 cells produced only those precipitation lines which corresponded with the inner lines obtained from the cytolytic infection. Similarly, hyperimmune rabbit antiserum against antigens extracted from the persistent infection lacked the antibody which was responsible for the outer lines of precipitation. Indirect immunofluorescence with acetone-fixed and unfixed cells using the homologous and heterologous sera confirmed the absence of antigens in the persistent infection and showed that an antigen is produced in the persistently infected cells which is either absent or present in very small amounts in cytolytically infected cells. Neutralization experiments and ether treatment suggested that the missing antigens in the persistent infection were the envelope components of foamy virus. It is proposed that the persistent infection has properties in common with some infections by RNA tumour viruses.